Sequential flow cytometry and fluorescence in situ hybridization for the study of formalin-fixed, paraffin-embedded breast cancer cells

Cancer Genetics and Cytogenetics
H F MarkB E Barker

Abstract

Both flow cytometry and fluorescence in situ hybridization (FISH) are useful techniques in the analysis of cancer tissues. When the two are used in the study of the same specimens, they are usually performed in parallel, separately. This is problematic where there is a scarcity of material, making completion of both studies impossible. Fluorescence in situ hybridization procedures that will utilize excess material discarded from flow cytometry would be advantageous. The present report describes an optimized protocol for performing sequential flow cytometry and FISH using formalin-fixed paraffin-embedded archival material. Although breast cancer tissues were used in this initial study, the protocol is applicable to other cancer tissues as well.

References

Nov 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D W HedleyE A Musgrove
Mar 1, 1993·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Z Gibas, A Talerman
Jun 1, 1996·Cancer Genetics and Cytogenetics·P CampomenosiG Fronza
Jan 1, 1996·Breast Cancer Research and Treatment·A AfifyH F Mark

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Citations

Aug 10, 1999·Luminescence : the Journal of Biological and Chemical Luminescence·P E Stanley, L J Kricka

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