Sequential gemcitabine and tamoxifen treatment enhances apoptosis and blocks transformation in bladder cancer cells.

Oncology Reports
Hisashi TakeuchiAshish M Kamat

Abstract

Bladder cancer is a common malignancy for which regional or metastatic disease is identified at diagnosis. The aim of this study was to determine whether tamoxifen (Tam), an estrogen receptor (ER) antagonist, can sensitize bladder cancer cell lines to gemcitabine (Gem) chemotherapy. ERα and ERβ protein levels were determined in each cell line using western blot analysis. The TCC-Sup, 5637, and RT4 bladder cancer cells were exposed to various concentrations and regimens of Tam or Gem alone or in combination. Cell viability and apoptosis were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and propidium iodide followed by flow cytometry. Apoptosis was then evaluated by western blot analysis. Treated TCC-Sup cells were subjected to soft agar colony formation assay to determine the cellular transformation. Western blot analysis results revealed ER expression in the three cell lines. TCC-Sup and 5637 cells treated with a combination of Tam and Gem had lower cell viabilities than those treated with Tam or Gem alone for 72 h in TCC-Sup and 5637. Compared with the other treatments, sequential Gem followed by Tam (Gem→Tam) treatment caused the largest increase in DNA fragmentation at 72 h in TCC-Sup cells....Continue Reading

References

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Citations

Jun 29, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Feihu JiTingmei Chen
Apr 5, 2016·Scientific Reports·Goodwin G JineshAshish M Kamat
Mar 12, 2017·Apoptosis : an International Journal on Programmed Cell Death·Rikiya TaokaAshish M Kamat
Nov 30, 2018·Virchows Archiv : an International Journal of Pathology·Philipp ErbenUNKNOWN BRIDGE Consortium e.V.
Oct 13, 2020·Biomolecules & Therapeutics·Huihui ZhuJingji Jin
Apr 3, 2021·Frontiers in Endocrinology·Takuro Goto, Hiroshi Miyamoto
Sep 29, 2021·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Josephine M Hyldgaard, Jørgen Bjerggaard Jensen

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Methods Mentioned

BETA
fluorescence-activated cell sorting
flow cytometry
electrophoresis

Software Mentioned

Ekuseru

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