Sequential gene expression changes in cancer cell lines after treatment with the demethylation agent 5-Aza-2'-deoxycytidine

Cancer
Makoto AraiTakenori Ochiai

Abstract

5-Aza-2'-deoxycytidine (5-AzaC) is known well for its demethylation effect and is a promising anticancer agent. However, to the authors' knowledge, serial changes in gene expression over time after 5-AzaC treatment have not been studied to date. To clarify the categories of genes that are up-regulated or down-regulated after 5-AzaC treatment, the authors surveyed the genes that had expression levels changed by 5-AzaC treatment in 6 hepatoma cell lines (Hep3B, HLE, Huh7, HepG2, PLC/PRF/5, and Huh6). Cell lines were grown in medium that contained 1 microM of 5-AzaC. Changes in messenger RNA levels were monitored from 24 hours up to 120 hours after 5-AzaC treatment using an in-house microarray that consisted of 4608 combinational DNAs. Using clustering analysis to identify the genes that had gradually changed expression levels and to exclude the substantial experimental noise by microarray analysis, the authors focused on 206 up-regulated genes and 248 down-regulated genes. According to their functional characterization, genes that were involved in the cytoskeleton and the extracellular matrix were enriched significantly in the up-regulated genes. Conversely, genes that were involved in metabolism were enriched significantly in th...Continue Reading

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Citations

Feb 19, 2009·Zygote : the Biology of Gametes and Early Embryos·Jian-Ning YuHong-Lin Liu
Feb 26, 2016·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Jana Elena KesselsPeter Uciechowski
May 19, 2009·BioFactors·Iwona Cicha, Margarete Goppelt-Struebe
Mar 6, 2017·International Journal of Oncology·Young Il KimHee Joo Lee
Aug 2, 2017·The Journal of Clinical Investigation·Francis O EnaneYogen Saunthararajah
Feb 6, 2020·Journal of Bioinformatics and Computational Biology·Peng LiBo Sun

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