Sequential injection chromatographic determination of paracetamol, caffeine, and acetylsalicylic acid in pharmaceutical tablets

Journal of Separation Science
Dalibor ŠatínskýAlberto N Araújo

Abstract

In this contribution, a new separation method for simultaneous determination of paracetamol, caffeine, acetylsalicylic acid, and internal standard benzoic acid was developed based on a novel reversed-phase sequential injection chromatography (SIC) technique with UV detection. A Chromolith Flash RP-18e, 25-4.6mm column (Merck, Germany) and a FIAlab 3000 system (USA) with an 8-port selection valve and a 5 mL syringe were used for sequential injection chromatographic separations in our study. The mobile phase used was acetonitrile-(0.01 M) phosphate buffer (10:90, v/v) pH 4.05, flow rate 0.6 mL min(-1). UV detection was at 210 and 230 nm. The validation parameters showed good results: linearity (r >0.999) for all compounds, detection limits in the range 0.3-0.8 microg mL(-1), repeatability (RSD) of peak heights between runs in the range 1.10-4.30% at three concentration levels and intra-day repeatability of the retention times in the range 0.28-0.43%. The analysis time was <6 min. The method was found to be applicable for the routine analysis of the active compounds paracetamol, caffeine, and acetylsalicylic acid in pharmaceutical tablets.

References

Mar 7, 2000·Journal of Pharmaceutical and Biomedical Analysis·A Ruiz MedinaA Molina-Diaz
Mar 15, 2001·Journal of Pharmaceutical and Biomedical Analysis·J T FranetaM B Aleksic
Aug 28, 2002·The Analyst·Claire E LenehanSimon W Lewis
Mar 1, 1940·California and Western Medicine·W A Bastedo

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Citations

Dec 5, 2012·Analytical and Bioanalytical Chemistry·Hana SklenářováPetr Solich
Jul 12, 2007·Journal of Separation Science·Stuart D ChambersCharles A Lucy
May 6, 2008·Analytical and Bioanalytical Chemistry·Xiaodong ShangGuanJianbin Zheng
Jan 22, 2020·Journal of Separation Science·Samuel W FosterJames P Grinias

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