Sequential muscle biopsies during a 6-h tracer infusion do not affect human mixed muscle protein synthesis and muscle phenylalanine kinetics.

American Journal of Physiology. Endocrinology and Metabolism
E VolpiBlake B Rasmussen

Abstract

Stable isotope tracer experiments of human muscle amino acid and protein kinetics often involve a sequential design, with the same subject studied at baseline and during an intervention. However, prolonged fasting and sequential muscle biopsies from the same area could theoretically affect muscle protein metabolism. The purpose of this study was to determine if sequential muscle biopsies and extended fasting significantly affect parameters of muscle protein and amino acid kinetics in six human subjects. After a 12-h overnight fast, a primed continuous infusion of L-[ring-(2)H(5)]phenylalanine was started. After 120 min, we took the first of a series of five hourly muscle biopsies from the same vastus lateralis to measure mixed muscle protein fractional synthetic rate. Furthermore, between 150-180, 210-240, and 330-360 min, we measured leg phenylalanine kinetics using the two-pool and the three-pool arteriovenous balance models. Tracer enrichments were at steady state, and muscle protein FSR and phenylalanine kinetics did not change throughout the experiment (P=not significant). We conclude that a 6-h tracer infusion during extended fasting (up to 18 h) with five sequential muscle biopsies from the same muscle do not affect basa...Continue Reading

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Citations

Mar 23, 2010·American Journal of Physiology. Endocrinology and Metabolism·Micah J DrummondBlake B Rasmussen
Nov 27, 2010·Journal of Applied Physiology·Gordon I SmithBettina Mittendorfer
Sep 11, 2012·Metabolism: Clinical and Experimental·Jared M DickinsonBlake B Rasmussen
Jan 3, 2012·Metabolism: Clinical and Experimental·Nicholas A BurdLuc J C van Loon
Dec 17, 2014·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Nicholas A BurdLuc J C van Loon
Mar 16, 2018·Journal of Applied Physiology·Jared M DickinsonChad C Carroll
Apr 3, 2014·American Journal of Physiology. Endocrinology and Metabolism·David M GundermannBlake B Rasmussen
Aug 10, 2020·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Jess A GwinStefan M Pasiakos

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