PMID: 8603080Mar 13, 1996Paper

Sequential ordered mechanism for the sodium-glutamate transport in intestinal brush border membrane vesicles

Biochimica Et Biophysica Acta
G Prezioso, V Scalera

Abstract

The glutamate/aspartate carrier localized in the brush-border membrane vesicles from enterocytes is known as a transport system catalyzing a sodium-substrate cotransport driven by the sodium gradient across the membrane. The kinetics of this transport system is studied by analogy with an enzymatic bi-substrate reaction. The results of this approach can be summarized as follows: (1) The dependence of the L-glutamate transport rate on the sodium concentration is sigmoidal, and the stoichiometry of the transport is 2 Na+/1 glutamate/1 carrier molecule. (2) The mechanism is sequential ordered, with L-glutamate binding after both the sodium cations. In addition, there is a very high degree of cooperativity between the two sodium binding sites.

Citations

Apr 7, 1997·Biochemical and Biophysical Research Communications·A MordrelleD Tomé
Aug 1, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·A MordrelleJ F Huneau

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