Sequential phosphorylation of Ser-10 on histone H3 and ser-139 on histone H2AX and ATM activation during premature chromosome condensation: relationship to cell-cycle phase and apoptosis

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Xuan HuangZbigniew Darzynkiewicz

Abstract

Histone H1 and H3 phosphorylation associated with chromatin condensation during mitosis has been studied extensively. Less is known on histone modifications that occur during premature chromosome condensation (PCC). The aim of the present study was to reveal the status of histone H3 and H2AX phosphorylation on Ser-10 and Ser-139, respectively, as well as ATM activation through phosphorylation on Ser-1981, during PCC, and relate these events to cell-cycle phase and to initiation of apoptosis. To induce PCC, A549 and HL-60 cells were exposed to the phosphatase inhibitor calyculin A (Cal A). Phosphorylation of histone H3 and H2AX as well as ATM activation were detected immunocytochemically concurrent with analysis of cellular DNA content and activation of caspase-3, a marker of apoptosis. The intensity of cellular fluorescence was measured by flow- or laser scanning cytometry. Induction of PCC led to rapid histone H3 phosphorylation, followed by activation of ATM and then H2AX phosphorylation in both, HL-60 and A549 cells. All these events occurred sequentially, prior to caspase-3 activation, and affected cells in all phases of the cell cycle. ATM activation and H2AX phosphorylation was seen during mitosis of A549 but not HL-60 ce...Continue Reading

References

Apr 1, 1977·The Journal of Cell Biology·Z DarzynkiewiczM R Melamed
Feb 5, 1973·Biochemical and Biophysical Research Communications·L R GurleyR A Tobey
Jul 8, 1980·Biochemistry·M H West, W M Bonner
Jan 25, 1994·Nucleic Acids Research·T H Thatcher, M A Gorovsky
Apr 16, 1998·The Journal of Biological Chemistry·E P RogakouW M Bonner
May 18, 1999·Experimental Cell Research·Z DarzynkiewiczM R Melamed
Jan 19, 2000·Nature·B D Strahl, C D Allis
Nov 4, 2000·Methods in Cell Biology·G JuanZ Darzynkiewicz
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·M B Kastan, D S Lim
May 4, 2001·Micron : the International Research and Review Journal for Microscopy·J Dobrucki, Z Darzynkiewicz
Aug 11, 2001·Science·T Jenuwein, C D Allis
Sep 26, 2001·The Journal of Biological Chemistry·S BurmaD J Chen
Sep 19, 2002·Radiation Research·Olga A SedelnikovaWilliam M Bonner
Jan 31, 2003·Nature·Christopher J Bakkenist, Michael B Kastan
Mar 4, 2003·Nature Reviews. Cancer·Yosef Shiloh
Apr 2, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Xuan HuangZbigniew Darzynkiewicz
Jul 10, 2004·Cell·Christopher J Bakkenist, Michael B Kastan
Aug 25, 2004·Chemistry & Biology·K Kristjánsdóttir, J Rudolph
Oct 2, 2004·Nature Reviews. Molecular Cell Biology·Jiri BartekJiri Lukas
Apr 23, 2005·Cell Cycle·Jirina BartkovaJiri Bartek
Jul 21, 2005·Molecular Biology of the Cell·Kirk J McManus, Michael J Hendzel
Aug 16, 2005·Cell Proliferation·Xuan HuangZbigniew Darzynkiewicz
Sep 1, 2005·Cancer Letters·Akihisa Takahashi, Takeo Ohnishi
Sep 13, 2005·Biochemical and Biophysical Research Communications·Yosuke IchijimaHirobumi Teraoka
Sep 27, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Akira KuroseZbigniew Darzynkiewicz

❮ Previous
Next ❯

Citations

Jun 19, 2009·European Biophysics Journal : EBJ·B HübnerT Cremer
May 7, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Hong ZhaoZbigniew Darzynkiewicz
Mar 1, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Zbigniew Darzynkiewicz
Jul 12, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Toshiki TanakaZbigniew Darzynkiewicz
Nov 10, 2012·Critical Reviews in Clinical Laboratory Sciences·Zbigniew DarzynkiewiczDonald Wlodkowic
Jan 26, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Malgorzata KajsturaZbigniew Darzynkiewicz
Jan 4, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Andrea Lisa HolmeShazib Pervaiz
Feb 13, 2009·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·J W J BergsN A P Franken
Jan 23, 2014·Cellular and Molecular Life Sciences : CMLS·Stéphanie Solier, Yves Pommier
Feb 18, 2011·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Kendra L Cann, Graham Dellaire
Jun 11, 2019·Frontiers in Molecular Neuroscience·Guobao LiTao P Zhong
Jun 24, 2010·International Journal of Radiation Biology·Thomas E SchmidBarbara Röper

❮ Previous
Next ❯

Related Concepts

Related Feeds

Ataxia telangiectasia

Ataxia telangiectasia is a rare neurodegenerative diseases caused by defects in the ATM gene, which is involved in DNA damage recognition and repair pathways. Here is the latest research on this autosomal recessive disease.

Ataxia telangiectasia (MDS)

Ataxia telangiectasia is a rare neurodegenerative diseases caused by defects in the ATM gene, which is involved in DNA damage recognition and repair pathways. Here is the latest research on this autosomal recessive disease.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis