Sequential recruitment of steroid receptor coactivator-1 (SRC-1) and p300 enhances progesterone receptor-dependent initiation and reinitiation of transcription from chromatin

Proceedings of the National Academy of Sciences of the United States of America
Z LiuB W O'Malley

Abstract

Employing a cell-free chromatin transcription system that recapitulates progesterone receptor (PR)-mediated transcription in vivo, we have investigated further the coactivator functions of steroid receptor coactivator-1 (SRC-1) in terms of its functional domains as well as cooperation with other coactivators in PR transactivation. By analyzing wild-type and mutant SRC-1 with liganded PR in the chromatin transcription system in vitro, the basic helix-loop-helix/Per-Arnt-Sim domain, the p300-binding domain, and the carboxyl-terminal region (containing the PR-binding site) of SRC-1 were shown to be important for PR transactivation. Although in context of a synthetic promoter its histone acetyltransferase activity was nonessential for PR-mediated transcription, SRC-1 was observed to act synergistically with p300 to enhance PR transactivation from chromatin. Moreover, SRC-1 and p300 were found to function cooperatively to increase the efficiency of productive transcription initiation and reinitiation. Further analysis of synergism between SRC-1 and p300 revealed an obligatory "sequential" recruitment of SRC-1 and p300 to liganded PR. Efficient recruitment of p300 required the presence of SRC-1. In addition, functional analysis of SR...Continue Reading

References

Jan 1, 1994·Annual Review of Biochemistry·M J Tsai, B W O'Malley
Jan 1, 1993·Molecular Endocrinology·D F Smith, D O Toft
Dec 15, 1995·Cell·D J MangelsdorfR M Evans
Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·C L SmithB W O'Malley
Dec 19, 1996·Nature·A J Bannister, T Kouzarides
Jan 1, 1997·Annual Review of Medicine·F CadepondE E Baulieu
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·G JensterB W O'Malley
Sep 20, 1997·Nature·T E SpencerB W O'Malley
Jun 16, 1999·Endocrine Reviews·N J McKennaB W O'Malley
Jun 26, 1999·Science·D ChenM R Stallcup
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·Z LiuB W O'Malley

❮ Previous
Next ❯

Citations

Mar 26, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Lan LiaoJianming Xu
Aug 29, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Michael R StallcupDagang Chen
Feb 13, 2009·The Journal of Biological Chemistry·Hany Abdel-HafizKathryn B Horwitz
Dec 20, 2011·Carcinogenesis·Charles GiardinaDaniel W Rosenberg
Jul 30, 2002·The EMBO Journal·Borja BelandiaMalcolm G Parker
Jul 12, 2003·Biology of Reproduction·Heather A MolendaMarc J Tetel
Aug 23, 2002·Molecular and Cellular Biology·Majdi S QutobJoseph Torchia
Feb 18, 2004·Molecular and Cellular Biology·Young-Ho LeeMichael R Stallcup
Mar 10, 2009·Biochemistry. Biokhimii︠a︡·L K SavinkovaN A Kolchanov
Nov 5, 2011·Molecular Endocrinology·Ying WangBert W O'Malley
Mar 16, 2012·International Journal of Biological Sciences·Claire A WalshJianming Xu
Sep 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Immaculata De VivoDavid J Hunter
Feb 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Maggie C LouieHong-Wu Chen
Mar 6, 2015·International Journal of Molecular Sciences·Ji-Long LiuYong-Sheng Fu
May 10, 2011·The International Journal of Biochemistry & Cell Biology·Nicole L Moore, Nancy L Weigel
Apr 30, 2009·Frontiers in Neuroendocrinology·Marc J TetelThierry D Charlier
Oct 16, 2015·Journal of Molecular Evolution·Yuan AnChaoyong James Yang
Sep 13, 2014·Journal of Cellular and Molecular Medicine·Francesca De AmicisSebastiano Andò
Apr 29, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Toshie Kanayasu-ToyodaTeruhide Yamaguchi
Apr 30, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Andrew P Dennis, Bert W O'Malley
Apr 29, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Andrew P DennisBert W O'Malley
Nov 16, 2004·Mechanisms of Ageing and Development·Xiaotao LiBert W O'Malley
Aug 18, 2006·Molecular Aspects of Medicine·Paolo AscenziMaria Marino
Mar 28, 2013·International Journal of Endocrinology·Monika Puzianowska-KuznickaJacek Polosak
Oct 31, 2003·American Journal of Obstetrics and Gynecology·Daniel W CramerImmaculata De Vivo
Mar 9, 2005·Trends in Biochemical Sciences·David M Lonard, Bert W O'Malley
Oct 9, 2016·The Journal of Steroid Biochemistry and Molecular Biology·Brenda Marquina-SánchezAliesha González-Arenas
Jan 28, 2004·The Journal of Biological Chemistry·Jeannine BotosCatharine L Smith
Apr 12, 2002·Endocrine Reviews·Cynthia A Heinlein, Chawnshang Chang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.