Sequential removal of Ca2+ from satellite tobacco necrosis virus. Crystal structure of two EDTA-treated forms

Journal of Molecular Biology
I MonteliusT Unge

Abstract

Two forms of EDTA-treated satellite tobacco necrosis virus (STNV) have been studied with X-ray crystallography methods. The crystals of both forms were isomorphous with native STNV crystals, and (FEDTA-Fnat) maps as well as (2FEDTA-Fnat) maps were calculated with phases from the native structure. The maps were based on partial data sets to 2.8 A resolution, and averaged using the 60-fold non-crystallographic symmetry. In the first crystal form, calcium ions were absent from one of the three sites in the icosahedral protein shell. The crystals were produced at pH 5.0 from a virus solution treated with EDTA at pH 6.5. The virions were not expanded, and no essential changes were seen in the protein shell. In the second crystal form, all calcium ions in the protein shell were absent. The virus material in these crystals had been subjected to treatment with EDTA at pH 8.0 before crystallization at pH 6.5. The high pH treatment caused degradation of the viral RNA. No expansion of the virion had occurred and all protein--protein contacts were retained. These results are compared with the previously presented low-resolution structure of slightly expanded STNV with intact RNA, where calcium ions from two sites were absent. The relevance...Continue Reading

References

Apr 1, 1977·Virology·A C DurhamM B Von Wechmar
Jul 8, 1988·Science·B A Wallace, K Ravikumar
May 20, 1988·Journal of Molecular Biology·I MonteliusT Unge
Dec 22, 1988·Biochimica Et Biophysica Acta·R DiPolo, L Beaugé
Jan 1, 1986·Progress in Biophysics and Molecular Biology·L Liljas
Jun 6, 1985·Nature·A Brisson, P N Unwin
Jul 25, 1982·Journal of Molecular Biology·L LiljasB Strandberg
Oct 1, 1987·Biophysical Journal·A M SilvaD J Goldstein

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Citations

Oct 1, 1991·International Journal of Biological Macromolecules·L Liljas
May 17, 2012·PLoS Computational Biology·Daniel S D LarssonDavid van der Spoel
Jan 20, 2018·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Natalia Martín-GonzálezPedro J de Pablo

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