Sequential rounds of RNA-dependent RNA transcription drive endogenous small-RNA biogenesis in the ERGO-1/Argonaute pathway.

Proceedings of the National Academy of Sciences of the United States of America
Jessica J VasaleDarryl Conte

Abstract

Argonaute (AGO) proteins interact with distinct classes of small RNAs to direct multiple regulatory outcomes. In many organisms, including plants, fungi, and nematodes, cellular RNA-dependent RNA polymerases (RdRPs) use AGO targets as templates for amplification of silencing signals. Here, we show that distinct RdRPs function sequentially to produce small RNAs that target endogenous loci in Caenorhabditis elegans. We show that DCR-1, the RdRP RRF-3, and the dsRNA-binding protein RDE-4 are required for the biogenesis of 26-nt small RNAs with a 5' guanine (26G-RNAs) and that 26G-RNAs engage the Piwi-clade AGO, ERGO-1. Our findings support a model in which targeting by ERGO-1 recruits a second RdRP (RRF-1 or EGO-1), which in turn transcribes 22G-RNAs that interact with worm-specific AGOs (WAGOs) to direct gene silencing. ERGO-1 targets exhibit a nonrandom distribution in the genome and appear to include many gene duplications, suggesting that this pathway may control overexpression resulting from gene expansion.

References

May 1, 1974·Genetics·S Brenner
Mar 31, 2000·Science·A GrishokC C Mello
Aug 15, 2002·Current Biology : CB·Femke SimmerRonald H A Plasterk
May 16, 2003·Current Biology : CB·Victor AmbrosDavid Jewell
Oct 22, 2003·Cell·Dianne S SchwarzPhillip D Zamore
Oct 22, 2003·Cell·Anastasia KhvorovaSumedha D Jayasena
Dec 13, 2006·Science·Titia SijenRonald H A Plasterk
Jul 3, 2007·Nature Structural & Molecular Biology·Suzanne R Lee, Kathleen Collins
Jul 31, 2007·Molecular Cell·Serafin U ColmenaresDanesh Moazed
Oct 19, 2007·Nature Reviews. Genetics·Elisabeth J Chapman, James C Carrington
Jan 17, 2009·Nature Reviews. Genetics·Megha Ghildiyal, Phillip D Zamore
Sep 8, 2009·Nature Methods·Marlon StoeckiusNikolaus Rajewsky
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Colin C ConineCraig C Mello

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Citations

Aug 13, 2011·Functional & Integrative Genomics·Sadegh Azimzadeh Jamalkandi, Ali Masoudi-Nejad
Nov 2, 2013·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Christopher J WedelesJulie M Claycomb
Nov 15, 2011·Parasitology·Jimmy J Zhuang, Craig P Hunter
Dec 20, 2011·Nature Structural & Molecular Biology·Caroline ThiviergeThomas F Duchaine
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Colin C ConineCraig C Mello
Jan 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Chi ZhangGary Ruvkun
Oct 17, 2012·DNA and Cell Biology·Julie M Claycomb
Jun 8, 2012·Genome Research·M Bryan WarfBrenda L Bass
Mar 14, 2012·PLoS Genetics·Katlin B MassirerAmy E Pasquinelli
Jun 15, 2011·PLoS Neglected Tropical Diseases·Johnathan J DalzellAaron G Maule
Jun 3, 2014·BMC Genomics·Jia-Jia WangDa-Wei Huang
Sep 27, 2014·Proceedings of the National Academy of Sciences of the United States of America·Aisa SakaguchiShawn Ahmed
Dec 20, 2013·Genes & Development·Sylvia E J FischerGary Ruvkun
Aug 4, 2012·RNA Biology·Chi Zhang, Gary Ruvkun
Apr 27, 2013·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Xuezhu Feng, Shouhong Guang
Jul 23, 2015·International Journal for Parasitology. Drugs and Drug Resistance·Ben-Wen LiGary J Weil
Apr 26, 2011·Current Biology : CB·Eleanor M Maine
Aug 2, 2012·The EMBO Journal·Maartje J LuteijnRené F Ketting
Mar 27, 2012·The Plant Journal : for Cell and Molecular Biology·Xianwei SongXiaofeng Cao
Aug 25, 2015·Journal of Integrative Plant Biology·Glykeria MermigkaKriton Kalantidis
Jul 19, 2013·Biochemical Society Transactions·Amy H Buck, Mark Blaxter
Aug 12, 2014·Biochemical Society Transactions·Monica J Piatek, Andreas Werner
Sep 24, 2013·Worm·Katlin B Massirer, Amy E Pasquinelli
Oct 24, 2014·Worm·Suzanne W HoogstrateL Basten Snoek
Feb 15, 2011·Developmental Cell·René F Ketting
Dec 24, 2013·Biochimica Et Biophysica Acta·Germano Cecere, Alla Grishok

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