Sequential statistical improvement of the liquid cultivation of Helicobacter pylori

Helicobacter
Rocio Duque-JamaicaAlba A Trespalacios

Abstract

Colonization of the gastric mucosa by Helicobacter pylori is one of the most important causes of acute and chronic gastric pathologies in humans. Achieving the growth of H. pylori in liquid media is of great importance in the development of clinical studies. In this study, we developed a sequential optimization strategy based on statistical models to improve the conditions of liquid culture of H. pylori. Four statistical models were sequentially used. First, a Box-Behnken design was used to select the best process conditions (shaking speed, inoculum concentration, and final volume of culture). Secondly, a general factorial design was used to evaluate the influence of adding gel blocks or gel beads (shape and composition). Then a D-optimal reduce design was carried out to allow the selection of the most influential factors in increasing the cell concentration (culture media components). Finally, another Box-Behnken design was used to optimize the concentration of the culture media components previously selected. After 12 hours of liquid culture a concentration of 25 x 10(8) cells per mL (9.4 log(10) cells per mL) of H. pylori was obtained, compared with a predicted 32 x 10(8) (9.5 log(10) cells per mL), which means between 1 and...Continue Reading

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Citations

Jan 16, 2014·Indian Journal of Microbiology·Viviana Sarria-AlfonsoRaúl A Poutou-Piñales
Aug 31, 2013·The Journal of Microbiology·Alba A TrespalaciosDavid Y Graham
Feb 10, 2016·Applied Nursing Research : ANR·O Adeyemi-AdelanwaJascinth L M Lindo
Jan 10, 2013·International Journal of Immunopathology and Pharmacology·L J BessaI Rocha
Jul 2, 2020·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Irene HortelanoMaría Antonia Ferrús

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