PMID: 9525604Apr 3, 1998Paper

Sequential steps in human immunodeficiency virus particle maturation revealed by alterations of individual Gag polyprotein cleavage sites

Journal of Virology
K WiegersHans-Georg Kräusslich

Abstract

Retroviruses are produced as immature particles containing structural polyproteins, which are subsequently cleaved by the viral proteinase (PR). Extracellular maturation leads to condensation of the spherical core to a capsid shell formed by the capsid (CA) protein, which encases the genomic RNA complexed with nucleocapsid (NC) proteins. CA and NC are separated by a short spacer peptide (spacer peptide 1 [SP1]) on the human immunodeficiency virus type 1 (HIV-1) Gag polyprotein and released by sequential PR-mediated cleavages. To assess the role of individual cleavages in maturation, we constructed point mutations abolishing cleavage at these sites, either alone or in combination. When all three sites between CA and NC were mutated, immature particles containing stable CA-NC were observed, with no apparent effect on other cleavages. Delayed maturation with irregular morphology of the ribonucleoprotein core was observed when cleavage of SP1 from NC was prevented. Blocking the release of SP1 from CA, on the other hand, yielded normal condensation of the ribonucleoprotein core but prevented capsid condensation. A thin, electron-dense layer near the viral membrane was observed in this case, and mutant capsids were significantly less...Continue Reading

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Citations

Jan 31, 2002·AIDS·H G Göttlinger
Oct 24, 2003·Proceedings of the National Academy of Sciences of the United States of America·F LiC T Wild
Mar 18, 2004·Reviews in Medical Virology·Catherine S Adamson, Ian M Jones
Jul 30, 2005·Journal of Virology·Mark P DoddingJonathan P Stoye
Oct 28, 2005·The Journal of Biological Chemistry·Jing ZhouChristopher Aiken
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Mar 1, 2007·Current Opinion in HIV and AIDS·Monique NijhuisCharles A B Boucher
Jan 29, 2011·Journal of Virology·Kristina ClemensSuzanne Sandmeyer
Dec 3, 2010·PLoS Pathogens·Lars-Anders CarlsonKay Grünewald
Mar 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Laura HilditchJonathan P Stoye
Feb 18, 2011·Journal of Virology·Siddhartha A K DattaAlan Rein
Apr 11, 2014·Journal of Virology·Matthew R EnglandRebecca C Craven
Oct 21, 2015·Antimicrobial Agents and Chemotherapy·Emiko UranoEric O Freed
Jul 20, 2016·Viruses·Chris Hellmund, Andrew M L Lever
Nov 3, 2016·Proceedings of the National Academy of Sciences of the United States of America·Lalit DeshmukhG Marius Clore
Apr 15, 2003·Journal of Virology·Uta K von SchwedlerWesley I Sundquist

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