Sequential Zonal Chondrogenic Differentiation of Mesenchymal Stem Cells in Cartilage Matrices

Tissue Engineering. Part a
Seyedsina MoeinzadehEsmaiel Jabbari

Abstract

The higher regenerative capacity of fetal articular cartilage compared with the adult is rooted in differences in cell density and matrix composition. We hypothesized that the zonal organization of articular cartilage can be engineered by encapsulation of mesenchymal stem cells in a single superficial zone-like matrix followed by sequential addition of zone-specific growth factors within the matrix, similar to the process of fetal cartilage development. The results demonstrate that the zonal organization of articular cartilage can potentially be regenerated using an injectable, monolayer cell-laden hydrogel with sequential release of growth factors.

References

Nov 17, 2001·Biomacromolecules·A I Van Den BulckeH Berghmans
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 7, 2002·Arthritis Research·David Eyre
Dec 17, 2002·Journal of Pediatric Surgery·Julie R FuchsDario O Fauza
Feb 28, 2003·In Vitro Cellular & Developmental Biology. Animal·Karl W KavalkovichFrank Barry
Feb 6, 2004·Journal of Cell Science·Gary P DowthwaiteCharles W Archer
May 11, 2004·Genes & Development·Haruhiko AkiyamaBenoit de Crombrugghe
Nov 23, 2006·Matrix Biology : Journal of the International Society for Matrix Biology·Alfred OpolkaSusanne Grässel
Apr 20, 2007·Molecular Biology of the Cell·Atsuko ShimoyamaToshiyuki Yoneda
Oct 18, 2007·Macromolecular Bioscience·James A HendersonEsmaiel Jabbari
Apr 25, 2008·Development·Kinglun Kingston MakYingzi Yang
Apr 30, 2008·Methods : a Companion to Methods in Enzymology·Simon R TewTimothy E Hardingham
Dec 20, 2008·Journal of Biomedical Materials Research. Part a·Peter BernsteinStefan Fickert
Feb 18, 2010·International Orthopaedics·Mazen FalahGershon Volpin
Aug 10, 2010·Current Topics in Developmental Biology·Véronique Lefebvre, Pallavi Bhattaram
Apr 27, 2011·Tissue Engineering. Part C, Methods·Clara R CorreiaJoão F Mano
Jul 5, 2011·Biophysical Journal·Laurent Bozec, Marianne Odlyha
Aug 17, 2011·Osteoarthritis and Cartilage·R MahmoodianS Siegler
Oct 22, 2011·Stem Cell Reviews and Reports·Ivana GadjanskiGordana Vunjak-Novakovic
Nov 11, 2011·Trends in Biotechnology·Nastaran Mahmoudifar, Pauline M Doran
Apr 7, 2012·The Orthopedic Clinics of North America·Facundo Las HerasKenneth P H Pritzker
Jul 24, 2012·Arthritis Research & Therapy·Andre F SteinertMaximilian Rudert
Aug 31, 2012·The Journal of the American Academy of Orthopaedic Surgeons·Jessica C RiveraAnthony E Johnson
Jul 17, 2014·The Journal of Biological Chemistry·Katsuhiko AmanoBeate Lanske
Jan 6, 2015·Tissue Engineering. Part a·Jetze VisserJos Malda
Jan 22, 2015·Arthritis & Rheumatology·Elena KozhemyakinaAndrew B Lassar
Mar 6, 2015·Clinical Medicine Insights. Arthritis and Musculoskeletal Disorders·Darryl BlalockJinxi Wang
May 31, 2015·Arthritis Research & Therapy·Alexandra KarystinouCosimo De Bari
Dec 15, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lei LiAndrei S Chagin

❮ Previous
Next ❯

Methods Mentioned

BETA
nuclear magnetic resonance
DNA assay
electrophoresis
NMR
transfection

Software Mentioned

Primer3
ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.