Sequentially acting SOX proteins orchestrate astrocyte- and oligodendrocyte-specific gene expression

EMBO Reports
Susanne KlumMaria Bergsland

Abstract

SOX transcription factors have important roles during astrocyte and oligodendrocyte development, but how glial genes are specified and activated in a sub-lineage-specific fashion remains unknown. Here, we define glial-specific gene expression in the developing spinal cord using single-cell RNA-sequencing. Moreover, by ChIP-seq analyses we show that these glial gene sets are extensively preselected already in multipotent neural precursor cells through prebinding by SOX3. In the subsequent lineage-restricted glial precursor cells, astrocyte genes become additionally targeted by SOX9 at DNA regions strongly enriched for Nfi binding motifs. Oligodendrocyte genes instead are prebound by SOX9 only, at sites which during oligodendrocyte maturation are targeted by SOX10. Interestingly, reporter gene assays and functional studies in the spinal cord reveal that SOX3 binding represses the synergistic activation of astrocyte genes by SOX9 and NFIA, whereas oligodendrocyte genes are activated in a combinatorial manner by SOX9 and SOX10. These genome-wide studies demonstrate how sequentially expressed SOX proteins act on lineage-specific regulatory DNA elements to coordinate glial gene expression both in a temporal and in a sub-lineage-speci...Continue Reading

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Citations

Jun 5, 2019·International Journal of Molecular Sciences·Jan WittstattMelanie Küspert
Jan 21, 2020·Glia·Hélène HirbecCarole Escartin
Jan 12, 2020·Glia·Martina de MajoErik M Ullian
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Jul 25, 2021·International Journal of Molecular Sciences·Federica CherchiElisabetta Coppi

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