Sequentially addressable dielectrophoretic array for high-throughput sorting of large-volume biological compartments.

Science Advances
A IsozakiK Goda

Abstract

Droplet microfluidics has become a powerful tool in precision medicine, green biotechnology, and cell therapy for single-cell analysis and selection by virtue of its ability to effectively confine cells. However, there remains a fundamental trade-off between droplet volume and sorting throughput, limiting the advantages of droplet microfluidics to small droplets (<10 pl) that are incompatible with long-term maintenance and growth of most cells. We present a sequentially addressable dielectrophoretic array (SADA) sorter to overcome this problem. The SADA sorter uses an on-chip array of electrodes activated and deactivated in a sequence synchronized to the speed and position of a passing target droplet to deliver an accumulated dielectrophoretic force and gently pull it in the direction of sorting in a high-speed flow. We use it to demonstrate large-droplet sorting with ~20-fold higher throughputs than conventional techniques and apply it to long-term single-cell analysis of Saccharomyces cerevisiae based on their growth rate.

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Citations

Oct 3, 2020·Micromachines·Endre Joachim Mossige, Atle Jensen
Jun 27, 2020·Micromachines·Hoon Suk Rho, Han Gardeniers

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Methods Mentioned

BETA
chip
fluorescence-activated sorting
fluorescence-activated droplet sorter
fluorescence-activated droplet

Software Mentioned

SADA
COMSOL Multiphysics

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