Sequential/parallel production of potential Malaria vaccines--A direct way from single batch to quasi-continuous integrated production

Journal of Biotechnology
Reiner LuttmannGesine Cornelissen

Abstract

An intensification of pharmaceutical protein production processes can be achieved by the integration of unit operations and application of recurring sequences of all biochemical process steps. Within optimization procedures each individual step as well as the overall process has to be in the focus of scientific interest. This paper includes a description of the development of a fully automated production plant, starting with a two step upstream followed by a four step downstream line, including cell clarification, broth cleaning with microfiltration, product concentration with ultrafiltration and purification with column chromatography. Recursive production strategies are developed where a cell breeding, the protein production and the whole downstream is operated in series but also in parallel, each main operation shifted by one day. The quality and reproducibility of the recursive protein expression is monitored on-line by Golden Batch and this is controlled by Model Predictive Multivariate Control (MPMC). As a demonstration process the production of potential Malaria vaccines with Pichia pastoris is under investigation.

References

Dec 1, 2000·Molecular Biotechnology·J M CreggD R Higgins
Sep 27, 2002·Current Opinion in Biotechnology·Geoff P Lin CereghinoJames M Cregg
Apr 8, 2011·Biotechnology Journal·Kai LoegeringReiner Luttmann
Apr 8, 2011·Biotechnology Journal·Jens FrickeReiner Luttmann
Oct 4, 2011·Biotechnology Advances·Eda Celik, Pınar Calık
May 31, 2013·Journal of the Royal Army Medical Corps·Michael J World
Dec 19, 2013·TheScientificWorldJournal
Oct 2, 2014·Journal of Chromatography. a·Jessica PaulGesine Cornelissen

❮ Previous
Next ❯

Citations

Feb 6, 2020·Biotechnology and Bioengineering·Fabian FeidlMassimo Morbidelli
Dec 12, 2019·Frontiers in Bioengineering and Biotechnology·Julian KoppChristoph Herwig

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.