PMID: 11929239Apr 4, 2002Paper

Sequestering of Eu(III) by a GAAA RNA tetraloop

Journal of the American Chemical Society
Claudius Mundoma, Nancy L Greenbaum

Abstract

The site-specific binding of metal ions maintains an important role in the structure, thermal stability, and function of folded RNA structures. RNA tetraloops of the "GNRA" family (where N = any base and R = any purine), which owe their unusual stability to base stacking and an extensive hydrogen bonding network, have been observed to bind metal ions having different chemical and geometric properties. We have used laser-induced lanthanide luminescence and isothermal titration calorimetry (ITC) to examine the metal-binding properties of an RNA stem loop of the GNRA family. Previous research has shown that a single Eu(III) ion binds the stem loop fragment in a highly dehydrated site with a K(d) of approximately 12 microM. Curve-fitting analysis of the broad luminescence excitation spectrum of Eu(III) upon complexation with the tetraloop fragment indicates the possibility of two microenvironments that do not differ in hydration number. Binding of Eu(III) to the loop was accompanied by positive enthalpic changes, consistent with energetic cost of removal of water molecules and suggesting that the binding is entropically driven. By comparison, binding of Mg(II) or Mn(II) to the RNA loop, or Eu(III) to the DNA analogue of the loop, w...Continue Reading

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Citations

Aug 15, 2006·Journal of the American Society for Mass Spectrometry·Jason W Kieltyka, Christine S Chow
Mar 28, 2003·Biochemical and Biophysical Research Communications·Thomas E Edwards, Snorri Th Sigurdsson
Jan 27, 2010·Journal of the American Chemical Society·Erich G Chapman, Victoria J DeRose
Oct 26, 2005·RNA·Meredith Newby LambertNils G Walter
Dec 4, 2003·Biophysical Journal·Joanna SarzynskaTadeusz Kulinski
Aug 10, 2004·Chembiochem : a European Journal of Chemical Biology·Gapian Bianké, Robert Häner
Jun 24, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Lars OpherdenAdrian Keller
Jan 21, 2004·Journal of Molecular Recognition : JMR·Matthew J Cliff, John E Ladbury
May 1, 2004·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Olga IranzoJanet R Morrow
Mar 29, 2011·Biopolymers·Meredith Newby Spano, Nils G Walter
Dec 18, 2012·Chemical Science·Victor Dokukin, Scott K Silverman
Feb 12, 2010·Bioconjugate Chemistry·Ching-Hui HuangJanet R Morrow
Jul 21, 2005·Bioconjugate Chemistry·Rolf Tona, Robert Häner

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