PMID: 45002Oct 18, 1979

Sequestration of adenosine in crude extract from mouse liver and other tissues

Biochimica Et Biophysica Acta
P M Ueland, J Saebø

Abstract

Adenosine (1 microM) was incubated in the presence of dialyzed crude tissue extract from mouse liver and its degradation determined. At high concentration of tissue extract, a fraction of adenosine was not metabolized. This phenomenon, termed sequestration of adenosine, was shown to be affected in the same way by the same factors (pH, salt, reducing agent and adenine) as those affecting the protection of adenosine against deamination in the presence of the purified cyclic AMP-adenosine binding protein/S-adenosylhomocysteinase from mouse liver (Saebø, J. and Ueland, P.M. (1979) Biochim. Biophys. Acta 587, 333--340). These data point to a role of this protein in the sequestration of adenosine in crude extract. The sequestration potency in crude extract could be determined by diluting the extract in the presence of a constant amount of adenosine deaminase added to the tissue extract. Under these conditions there was linearity of adenosine not available for degradation versus the concentration of tissue extract, and a total recovery of the sequestration potency of purified binding protein added to the crude extract was observed. The tissue level of the cyclic AMP-adenosine binding protein/S-adenosylhomocysteinase in mouse liver was...Continue Reading

Citations

Jan 1, 1984·Pflügers Archiv : European journal of physiology·F L BelloniR M Berne
Jan 1, 1981·The International Journal of Biochemistry·S O Døskeland, D Ogreid
Oct 1, 1987·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·T S ParkR M Berne
Dec 1, 1988·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·T S ParkR M Berne
May 1, 1982·Circulation Research·R A OlssonC R Steinhart
Mar 1, 1987·Journal of Autonomic Pharmacology·P DorigoI Maragno
May 1, 1986·Basic Research in Cardiology·K J HenrichsW Schaper
Oct 1, 1984·Archives internationales de physiologie et de biochimie·J AussedatA Rossi

Related Concepts

Adenocard
Cyclic AMP, (R)-Isomer
Carrier Proteins
Bos indicus
Hydrogen-Ion Concentration
Hydrolase
Liver
Tissue Specificity
S-Adenosylhomocysteine
Tissue Extracts

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