Sequestration of amitriptyline by liposomes

Journal of Colloid and Interface Science
Marissa S Fallon, Anuj Chauhan

Abstract

We study the uptake of amitriptyline, which is a common cause of overdose-related fatalities, in aqueous solutions by 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) liposomes and liposomes composed of a mixture of DMPC and 1,2-dioleoyl-sn-glycero-3-[phospho-rac(1-glycerol)] (DOPG) lipids. The effect of drug concentration, liposomal charge, pH, salt, and protein presence on the drug uptake is investigated using two different methodologies, a precipitation and a centrifugation method. Furthermore, the time scale of the drug uptake is studied through qualitative observations at high pH and through conductivity measurements at neutral pH and found to be <5 s. The results of the quantitative studies show that the fractional drug uptake decreases with increasing drug concentration, and for a given concentration it increases with the pH and decreases in the presence of salt. We find that a larger amount of drug is sequestered by negatively charged liposomes (those containing DOPG) than liposomes with no net charge (DMPC). We speculate that the mechanism of drug uptake is due to both electrostatic interactions as well as hydrophobic effects. The fractional uptake by DMPC:DOPG in a 70:30 ratio is as high as 95% in water and about 90...Continue Reading

References

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Citations

Sep 18, 2013·Journal of Colloid and Interface Science·Guocheng LvWei-Teh Jiang
Jul 26, 2008·Nature Nanotechnology·Jean-Christophe Leroux
Jan 22, 2009·Journal of Pharmaceutical Sciences·Brett A Howell, Anuj Chauhan
May 3, 2008·Journal of Pharmaceutical Sciences·Giulia BonacucinaGiovanni F Palmieri
Jan 17, 2012·Acta Biomaterialia·Todd HoareDaniel S Kohane
May 27, 2008·Journal of Colloid and Interface Science·Brett Howell, Anuj Chauhan
Dec 14, 2007·Journal of Colloid and Interface Science·Brett Howell, Anuj Chauhan
Dec 25, 2007·Colloids and Surfaces. B, Biointerfaces·Monica A James-SmithDinesh O Shah
Nov 28, 2006·Biomaterials·Anand Babu DhanikulaJean-Christophe Leroux
Jun 19, 2015·Advanced Drug Delivery Reviews·Robert Damitz, Anuj Chauhan
Oct 23, 2009·Academic Emergency Medicine : Official Journal of the Society for Academic Emergency Medicine·Grant Cave, Martyn Harvey
Jan 13, 2009·Academic Emergency Medicine : Official Journal of the Society for Academic Emergency Medicine·Martyn HarveyKerry Hoggett
Oct 17, 2013·Academic Emergency Medicine : Official Journal of the Society for Academic Emergency Medicine·Grant CaveAnuj Chauhan
Apr 25, 2015·Academic Emergency Medicine : Official Journal of the Society for Academic Emergency Medicine·Michael R FettiplaceGuy Weinberg
Dec 9, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Michael R FettiplaceGuy Weinberg
Apr 15, 2016·The Journal of Physical Chemistry. B·Aruna K MoraSukhendu Nath
Jul 29, 2011·Nanomedicine·Lauren M GrahamSang Bok Lee
Oct 14, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Brett A Howell, Anuj Chauhan
Nov 12, 2010·ACS Nano·Nicolas BertrandJean-Christophe Leroux
Feb 7, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Monica A James-SmithDinesh O Shah

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