Sera with anti-enteric neuronal antibodies from patients with irritable bowel syndrome promote apoptosis in myenteric neurons of guinea pigs and human SH-Sy5Y cells

Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society
Wenjuan FanXiucai Fang

Abstract

Sera anti-enteric neuronal antibodies (AENA), neuronal inflammation, and degeneration in myenteric plexus in patients with irritable bowel syndrome (IBS) were reported. Effects of sera AENA in patients with IBS are unclear. Patients with IBS met Rome III criteria were enrolled. Controls included healthy subjects (HS) and patients with slow transit functional constipation, inflammatory bowel disease, chronic intestinal pseudo-obstruction, and autoimmune diseases. Indirect immunofluorescence was used to detect AENA. Anti-enteric neuronal antibodies intensities were termed as "1" = weak fluorescence (mild positive); "2" = moderate fluorescence (moderate positive); "3" = very high fluorescence (intensive positive). Intensities of ≥1 were defined as positive and ≥2 were defined as obvious positive. Cultured myenteric neurons of small intestine from guinea pigs and human SH-Sy5Y cells were incubated with fetal bovine serum (FBS), HS sera, or IBS sera with or without AENA. Indirect immunofluorescence with anti-PGP9.5/DAPI/anti-active caspase-3 or TUNEL, Western blot, and flow cytometry were used to detect apoptosis. Overall, 293 patients with IBS were enrolled (41.7 ± 11.5 years). AENA-positive and obvious positive rates in IBS were h...Continue Reading

References

Jan 1, 1997·Annual Review of Medicine·J B Posner, J O Dalmau
Jul 27, 2000·European Journal of Gastroenterology & Hepatology·J D Wood
Feb 15, 2002·Scandinavian Journal of Gastroenterology·C H KnowlesJ E Martin
Jun 11, 2002·Gastroenterology·Vinton S ChadwickIan Wilson
Aug 20, 2002·Journal of Clinical Gastroenterology·Jackie D Wood
Jul 10, 2003·Gastroenterology·Roberto De GiorgioRoberto Corinaldesi
Feb 1, 1960·Journal of Neurology, Neurosurgery, and Psychiatry·M HAMILTON
Jun 10, 2004·Gastroenterology·Roberto De GiorgioGary M Mawe
Mar 16, 2007·Developmental Biology·Meri LähteenmäkiMatti S Airaksinen
Jul 17, 2007·Current Opinion in Immunology·Jerry M Adams, Suzanne Cory
Oct 6, 2007·Scandinavian Journal of Gastroenterology·Hans TörnblomGreger Lindberg
Mar 14, 2008·Current Molecular Medicine·Kerstin ReimersPeter M Vogt
Dec 19, 2008·Expert Review of Gastroenterology & Hepatology·Cesare CremonGiovanni Barbara
Feb 19, 2009·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·L OhmanM Simrén
Aug 5, 2009·Inflammatory Bowel Diseases·Charles N BernsteinGillian Watermeyer
Dec 22, 2010·Journal of Crohn's & Colitis·Gabrio BassottiBruno Salerni
Dec 25, 2010·Digestive Diseases and Sciences·Sean J PittockG Richard Locke
Jul 1, 2011·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·B OhlssonG N Fredrikson
Feb 11, 2012·Journal of Neurogastroenterology and Motility·Jackie D WoodWilliam E Whitehead
May 18, 2012·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·S LudidiA A M Masclee
Jun 1, 1994·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·M HananiJ D Wood
May 15, 2015·Frontiers in Immunology·Romana Höftberger
Dec 10, 2015·Journal of Neurogastroenterology and Motility·Shan LiMeiyun Ke
Jun 9, 2016·The American Journal of Gastroenterology·Sean M P BennetLena Öhman
Dec 3, 2016·Scientific Reports·Qin LiMichael Schemann
May 26, 2017·Nature Reviews. Rheumatology·David S Pisetsky
Aug 30, 2017·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·M BashashatiG Barbara

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.