SERCA Activity Controls the Systolic Calcium Increase in the Nucleus of Cardiac Myocytes

Frontiers in Physiology
Tobias-Oliver Kiess, Jens Kockskämper

Abstract

In cardiomyocytes, nuclear calcium is involved in regulation of transcription and, thus, remodeling. The cellular mechanisms regulating nuclear calcium, however, remain elusive. Therefore, the aim of this study was to identify and characterize the factors that regulate nuclear calcium in cardiomyocytes. We focused on the roles of (1) the cytoplasmic calcium transient (CaT), (2) the sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA), and (3) intracellular calcium stores for nuclear calcium handling. Experiments were performed on rat ventricular myocytes loaded with Fluo-4/AM. Subcellularly resolved CaTs were visualized using confocal microscopy. The cytoplasmic CaT was varied by reducing extracellular calcium (from 1.5 to 0.3 mM) or by exposure to isoprenaline (ISO, 10 nM). SERCA was blocked by thapsigargin (5 μM). There was a strict linear dependence of the nucleoplasmic CaT on the cytoplasmic CaT over a wide range of calcium concentrations. Increasing SERCA activity impaired, whereas decreasing SERCA activity augmented the systolic calcium increase in the nucleus. Perinuclear calcium store load, on the other hand, did not change with either 0.3 mM calcium or ISO and was not a decisive factor for the nucleoplasmic CaT. T...Continue Reading

References

May 1, 1992·Cell Calcium·A WrzosekM Chiesi
Jan 1, 1995·Annual Review of Biochemistry·L I Davis
May 28, 1998·Cell Calcium·M A LeeL Stehno-Bittel
Jul 2, 1999·Biophysical Journal·O Keminer, R Peters
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·M J BerridgeM D Bootman
Jan 24, 2002·Nature·Donald M Bers
Sep 18, 2002·Journal of Molecular and Cellular Cardiology·Lars S Maier, Donald M Bers
Jul 3, 2003·Nature Reviews. Molecular Cell Biology·Michael J BerridgeH Llewelyn Roderick
Dec 6, 2003·Circulation Research·Benjamin J WilkinsJeffery D Molkentin
Jul 1, 2004·Journal of Cell Science·Oleg Gerasimenko, Julia Gerasimenko
Mar 3, 2006·The Journal of Clinical Investigation·Xu WuDonald M Bers
Aug 29, 2006·Nature Reviews. Molecular Cell Biology·Joerg Heineke, Jeffery D Molkentin
Apr 21, 2007·Annals of the New York Academy of Sciences·Robert W Ledeen, Gusheng Wu
Sep 1, 2007·The Journal of Physiology·Aleksey V ZimaLothar A Blatter
Jul 8, 2008·Journal of Molecular and Cellular Cardiology·Jens KockskämperMartin D Bootman
Jul 3, 2009·Journal of Cell Science·Martin D BootmanH Llewelyn Roderick
Dec 15, 2010·Journal of Molecular and Cellular Cardiology·Matias EscobarClara Franzini-Armstrong
May 18, 2011·Biophysical Journal·Senka LjubojevićJens Kockskämper
Dec 12, 2012·Frontiers in Physiology·Alejandro Domínguez-RodríguezAna M Gómez
Oct 21, 2014·Journal of Cardiovascular Pharmacology·Senka Ljubojevic, Donald M Bers
Mar 27, 2016·Cardiovascular Research·Katharina MederleHayo Castrop
Oct 25, 2016·Journal of Molecular and Cellular Cardiology·Adonis Z WuZhenhui Chen
Nov 7, 2016·Journal of Molecular and Cellular Cardiology·Jelena PlačkićJens Kockskämper
Oct 1, 2017·Circulation Research·Matthias DewenterJohannes Backs
Jul 11, 2018·Methods in Molecular Biology·Jelena Plačkić, Jens Kockskämper
Oct 8, 2018·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Silvana Olivares-FlorezOliver Ritter

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Citations

Aug 21, 2019·International Journal of Molecular Sciences·Afonso Caricati-NetoFrancisco Sandro Menezes-Rodrigues
Jan 23, 2021·Journal of Biochemistry·Mahira SafaralizadeBing Zhao
Oct 30, 2021·Pharmaceutical Biology·Piao ZhouJing Guo

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Methods Mentioned

BETA
confocal microscopy

Software Mentioned

ImageJ
GraphPad Prism

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