PMID: 8613703Apr 1, 1996Paper

Serglycin-binding proteins in activated macrophages and platelets

Journal of Leukocyte Biology
S O KolsetE Ruoslahti

Abstract

The major proteoglycan in macrophages and platelets is the chondroitin sulphate proteoglycan serglycin. To study the biological role of serglycin, its binding to secreted and cell-associated proteins from macrophages and blood platelets was examined. Affinity chromatography with serglycin-Sepharose and chondroitin sulphate-Sepharose was used to isolate proteoglycin-binding proteins from macrophages and platelets. Antibodies against human macrophage inflammatory protein-1 alpha (MIP-1 alpha) precipitated a 14-kDa 35S-methionine-labeled protein among the chondroitin sulfate binding proteins secreted from the macrophage-like U937 cells after stimulation. Two proteins from murine macrophage J774 cells with molecular masses of approximately 10 and 14 kDa were precipitated by an antiserum against the murine MIP-1 alpha. Protein sequencing of fragments obtained by trypsin digestion of a 14-kDa chondroitin sulfate-binding protein from cell extracts of stimulated U937 cells revealed 100% homology with lysozyme, a bacteriolytic enzyme. Fragment of one other protein with approximate molecular mass of 8 kDa showed high homology with bone morphogenetic protein. Inhibition studies showed that chondroitin 6-sulfate inhibited the bacteriolytic...Continue Reading

Citations

Jun 25, 1998·Glycobiology·M A KulsethS O Kolset
Mar 25, 2008·The Journal of Biological Chemistry·Nabin MallaJan-Olof Winberg
Dec 24, 2015·PloS One·Trine M ReineSvein O Kolset
May 29, 2016·Biointerphases·Brooke L FarrugiaMegan S Lord
Jan 25, 2017·The Journal of Biological Chemistry·Megan S LordJohn M Whitelock
Apr 17, 2020·Frontiers in Immunology·Helena CrijnsPaul Proost
Sep 10, 2003·Journal of Leukocyte Biology·Peter LemanskyAndrej Hasilik
Dec 18, 2008·Journal of Leukocyte Biology·Sara WernerssonMagnus Abrink
Apr 14, 2015·Immunity, Inflammation and Disease·Ingrid Benedicte M KolsethSvein Olav Kolset
Oct 23, 1997·The Journal of Biological Chemistry·N Toyama-SorimachiM Miyasaka
Apr 23, 1999·The Journal of Biological Chemistry·F PetersenD Spillmann
Jan 20, 2006·The FEBS Journal·Lillian ZernichowSvein O Kolset
Oct 3, 2007·Glycoconjugate Journal·Svein Olav Kolset, Lillian Zernichow
Feb 22, 2012·The Journal of Biological Chemistry·Mary Y ChangThomas N Wight
Mar 10, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Brooke L FarrugiaJohn M Whitelock
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Dec 23, 2006·Journal of Leukocyte Biology·Helene PiccardGhislain Opdenakker
May 16, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ha Na KimMegan S Lord
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