Serial Analysis of Gene Expression: Applications in Human Studies

Journal of Biomedicine & Biotechnology
Renu Tuteja, Narendra Tuteja

Abstract

Serial analysis of gene expression (SAGE) is a powerful tool, which provides quantitative and comprehensive expression profile of genes in a given cell population. It works by isolating short fragments of genetic information from the expressed genes that are present in the cell being studied. These short sequences, called SAGE tags, are linked together for efficient sequencing. The frequency of each SAGE tag in the cloned multimers directly reflects the transcript abundance. Therefore, SAGE results in an accurate picture of gene expression at both the qualitative and the quantitative levels. It does not require a hybridization probe for each transcript and allows new genes to be discovered. This technique has been applied widely in human studies and various SAGE tags/SAGE libraries have been generated from different cells/tissues such as dendritic cells, lung fibroblast cells, oocytes, thyroid tissue, B-cell lymphoma, cultured keratinocytes, muscles, brain tissues, sciatic nerve, cultured Schwann cells, cord blood-derived mast cells, retina, macula, retinal pigment epithelial cells, skin cells, and so forth. In this review we present the updated information on the applications of SAGE technology mainly to human studies.

Citations

Mar 17, 2007·Functional & Integrative Genomics·Rebecca PooleKeith J Edwards
Jun 1, 2005·Trends in Parasitology·David P Knox, Philip J Skuce
Nov 7, 2006·BMC Bioinformatics·Rodrigo MaligFrancisco Melo
Nov 9, 2006·Comparative Hepatology·Nicholas A ShackelGeoffrey W McCaughan
Jul 27, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Renu Tuteja, Narendra Tuteja
Jul 8, 2008·Acta Biochimica Et Biophysica Sinica·Zhonghua ZhouBoxiong Zhong

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
biopsy
electrophoresis
PCR

Software Mentioned

lite
SAGE

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