PMID: 15241239Jul 9, 2004Paper

Serial analysis of gene expression in neurofibromatosis type 2-associated vestibular schwannoma

Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology
Stacey L HalumP Ashley Wackym

Abstract

The genesis, morphology, and growth characteristics of vestibular schwannomas are determined by genetic alterations which vary gene transcript expression and this transcript expression can be qualitatively and quantitatively evaluated using the SAGE technique. By use of such technique, gene products with tumorigenic potential may be identified, providing insight and targets for future study. Serial analysis of gene expression (SAGE) is a powerful new technique that allows detailed qualitative and quantitative evaluation of cellular gene transcript expression. Tissue in limited quantity (5 x 10 to 2 x 10 cells) may be analyzed by a modified version of SAGE called microSAGE. Application of SAGE or microSAGE to study vestibular schwannoma gene expression has not been previously reported. Fresh, vestibular schwannoma specimen from an individual with the diagnosis of neurofibromatosis type 2 was attained intraoperatively and maintained in a sealed container at -80degreesC until the time of analysis. The tissue was processed according to the microSAGE protocol, using 180 mg of vestibular schwannoma as starting material. The protocol resulted in the generation and sequencing of a tag library involving 458 tags representing 277 differe...Continue Reading

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Citations

Aug 17, 2005·Audiology & Neuro-otology·Joseph P RochePaul Popper
Dec 1, 2009·Journal of Neurosurgery·Michael E SughrueAndrew T Parsa
Mar 10, 2007·Hearing Research·David R FriedlandPaul Popper
Aug 15, 2006·Neuroscience·D R FriedlandJ A Cioffi
Mar 22, 2006·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·David R FriedlandJoseph A Cioffi

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