Serial analysis of gene expression of esophageal squamous cell carcinoma: ADAMTS16 is upregulated in esophageal squamous cell carcinoma

Cancer Science
Naoya SakamotoWataru Yasui

Abstract

Esophageal squamous cell carcinoma (ESCC) is one of the most common malignancies worldwide. To identify potential diagnostic markers for ESCC and therapeutic targets for ESCC, we used Serial Analysis of Gene Expression (SAGE) on one ESCC sample. We obtained a total of 14,430 tags, including 5765 that were unique. By comparing SAGE tags from the ESCC sample with those from normal human squamous esophagus, we found several genes that were differentially expressed between ESCC and normal squamous esophagus. Among these, we focused on the ADAM metallopeptidase with thrombospondin type 1 motif, 16 (ADAMTS16) gene because quantitative RT-PCR analysis showed a high level of ADAMTS16 expression in eight out of 20 ESCC samples (40%), but not in 15 kinds of normal tissues. Western blot analysis also showed upregulation of ADAMTS16 protein in ESCC tissues. Furthermore, ADAMTS16 protein was detected in culture media from the TE5 esophageal cancer cell line. Knockdown of ADAMTS16 in TE5 cells inhibited both cell growth and invasion ability. Our present SAGE data provide a list of genes potentially associated with ESCC. ADAMTS16 could be a novel diagnostic and therapeutic target for ESCC.

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Citations

Mar 19, 2011·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Naohide OueWataru Yasui
Jun 7, 2014·Journal of Cellular Biochemistry·Zanhui JiaHong Zhang
Jul 17, 2015·Cancer Biotherapy & Radiopharmaceuticals·Ozlem CakmakKadir Demircan
May 12, 2015·Cancer Epidemiology·Matteo Di MasoJerry Polesel
Nov 26, 2013·Best Practice & Research. Clinical Anaesthesiology·Caroline P LeErica K Sloan
Aug 19, 2011·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Tetsutaro HayashiWataru Yasui
Apr 24, 2020·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Naoya SakamotoWataru Yasui

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