Oct 20, 1995

Serial analysis of gene expression

Science
V E VelculescuK W Kinzler

Abstract

The characteristics of an organism are determined by the genes expressed within it. A method was developed, called serial analysis of gene expression (SAGE), that allows the quantitative and simultaneous analysis of a large number of transcripts. To demonstrate this strategy, short diagnostic sequence tags were isolated from pancreas, concatenated, and cloned. Manual sequencing of 1000 tags revealed a gene expression pattern characteristic of pancreatic function. New pancreatic transcripts corresponding to novel tags were identified. SAGE should provide a broadly applicable means for the quantitative cataloging and comparison of expressed genes in a variety of normal, developmental, and disease states.

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Mentioned in this Paper

HUMTRPSGNB
SPINK1
HUMPANMU
HUMTNFRII
HUMPNLIP
HUMEGFRBB3
X67318
Gene Expression
Z11692
HUMPPE

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