Serial lectin affinity chromatography demonstrates altered asparagine-linked sugar chain structures of gamma-glutamyltransferase in human renal cell carcinoma

Journal of Chromatography. B, Biomedical Applications
K I YoshidaY Ueda

Abstract

Differences between human renal cortex and human renal cell carcinoma (RCC) in asparagine (Asn)-linked sugar chain structures of gamma-glutamyltransferase (GGT) were investigated by using a serial lectin affinity chromatographic technique. The relative amounts of GGT which passed through the concanavalin A (Con A) column but bound to the phytohaemagglutinin E column, were significantly decreased in RCC, but there were significant increases in the relative amounts of GGT which bound weakly to the Con A column and passed through the pea lectin (PSA) column, and bound strongly to the Con A column and bound to the wheat germ agglutinin column in RCC compared with those of the normal renal cortex. A significant correlation was observed in RCC between nuclear grade and relative amount of GGT which bound weakly to the Con A column and passed through the PSA column. The findings indicate that Asn-linked sugar chain structures are altered in RCC and suggest that studies of qualitative differences of sugar chain structures of GGT might lead to a useful diagnostic tool for human RCC.

References

Aug 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S S Tate, A Meister
Jan 1, 1976·Annual Review of Biochemistry·A Meister, S S Tate
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S Jaken, M Mason
Sep 15, 1989·Clinica Chimica Acta; International Journal of Clinical Chemistry·K AraiY Sakagishi
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·Y LapercheG Guellaën
Jan 1, 1983·Journal of Biochemistry·K YamashitaA Kobata
Sep 1, 1983·Archives of Biochemistry and Biophysics·K YamashitaA Kobata
Oct 1, 1982·The American Journal of Surgical Pathology·S A FuhrmanC Limas
Nov 1, 1960·Archives of Biochemistry and Biophysics·J A GOLDBARGA M RUTENBURG

❮ Previous
Next ❯

Citations

Nov 6, 2001·Journal of Biochemical and Biophysical Methods·P R Satish, A Surolia
Jan 26, 2010·Journal of Separation Science·Kishore K R Tetala, Teris A van Beek
Jan 31, 2002·Journal of Chromatography. B, Biomedical Sciences and Applications·S SumiK Yoshida
Oct 29, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·M CaronR Joubert-Caron
Dec 2, 2004·Analytical Chemistry·Xiuli MaoBingcheng Lin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.