Serial passage of MC3T3-E1 cells down-regulates proliferation during osteogenesis in vitro

Cell Proliferation
W J PetersonD T Yamaguchi

Abstract

Generally, fibroblast-like cells and other types of human cells have been used to demonstrate the principles of replicative senescence in vitro and in vivo. These cells go through three stages of proliferation, including vigorous proliferation, declining proliferation and quiescence or no proliferation. Any variation of this process occurring in osteoprogenitor cells may offer insight into the mechanism of age-related osteopaenia that predisposes individuals to osteoporosis and bone fractures. We selected MC3T3-E1 cells derived from mouse calvaria to study the mechanism of replicative senescence of pre-osteogenic cells because: (i) these cells constitute a well-known model for studying osteogenesis in vitro; (ii) they undergo a developmental sequence of proliferation and differentiation similar to primary cells in culture; and (iii) they show signs of replicative senescence. These cells were aged by multiple passaging before their use for studying growth kinetics and the effects of population density, effect of extracellular matrix (ECM), size and phases of the cell cycle. Our results show that (i) MC3T3-E1 cells go through the first two stages of proliferation in a manner similar to human cells, but escape the quiescent phase;...Continue Reading

References

Jun 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·L D QuarlesR J Wenstrup
Jul 1, 1993·Physiological Reviews·V J Cristofalo, R J Pignolo
Dec 1, 1996·Cell Proliferation·W J Peterson, D T Yamaguchi
Feb 16, 2002·Cellular and Molecular Life Sciences : CMLS·A J Obaya, J M Sedivy

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Citations

Nov 26, 2011·Cell Stress & Chaperones·Eunna Chung, Marissa Nichole Rylander
Oct 2, 2008·Plastic and Reconstructive Surgery·Artur GevorgyanChristopher R Forrest
Aug 1, 2013·Tissue Engineering. Part C, Methods·Xiang-Zhen YanSanne K Both
May 16, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Nabanita S DattaLaurie K McCauley
Aug 29, 2013·Journal of Biomedical Materials Research. Part a·E M CzekanskaJ S Hayes
Jun 9, 2021·European Journal of Dentistry·Charukrit LilakhunakonSomying Patntirapong
Sep 19, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Ruili LiuJiandong Ding

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