Abstract
When a new separation problem is faced with high-performance liquid chromatography (HPLC), the analysis is addressed conventionally with a single column, trying to find out a single experimental condition aimed to resolve all compounds. However, in practice, the system selectivity may be insufficient to achieve full resolution. When a separation fails, the usual practice consists of introducing drastic changes in the chromatographic system (e.g. use of another column, solvent or pH). An alternative solution is taking benefit of the combined separation capability of two or more columns, which can be attained in multiple ways, such as diverse modalities of two-dimensional HPLC, or mono-dimensional HPLC with serial or parallel columns. In this work, the separation performance offered by the serial coupling of columns of different nature and length, operated at varying mobile phase composition in isocratic elution, is compared with the results offered by parallel columns. The resolution capability of both approaches is characterised through the limiting peak purities. It is demonstrated that serial columns of different lengths perform as new columns that increase enormously the probabilities of success. The potential of the approac...Continue Reading
References
Feb 3, 2000·Analytical Chemistry·Y Mao, P W Carr
May 24, 2000·Journal of Chromatography. a·G Vivó-TruyolsM C García-Alvarez-Coque
Mar 28, 2006·Journal of Chromatography. a·J R Torres-Lapasió, M C García-Alvarez-Coque
May 15, 2007·Journal of Chromatography. a·Sz NyiredyL Szepesy
Feb 12, 2010·Analytical Chemistry·Maarten De BeerPat Sandra
Jul 26, 2011·Journal of Chromatography. a·A OrtínM C García-Álvarez-Coque
Feb 7, 2012·Journal of Chromatography. a·C Ortiz-BolsicoM C García-Álvarez-Coque
Jul 19, 2012·Journal of Separation Science·Filippo BedaniHans-Gerd Janssen
Feb 13, 2013·Journal of Chromatography. a·C Ortiz-BolsicoM C García-Álvarez-Coque
Mar 27, 2013·The Analyst·Kai ChenPat Sandra
Jul 13, 2013·Journal of Chromatography. a·C Ortiz-BolsicoM C García-Álvarez-Coque
Mar 26, 2014·Analytical Chemistry·Chunlei WangYingru Zhang
Jun 4, 2014·Journal of Chromatography. a·C Ortiz-BolsicoM C García-Alvarez-Coque
Jul 30, 2014·Analytica Chimica Acta·Michelle Camenzuli, Peter J Schoenmakers
Aug 26, 2014·Journal of Chromatography. a·A Escrig-DoménechG Ramis-Ramos
Citations
Dec 1, 2015·Bioanalysis·Hao WangJingkai Gu
May 9, 2016·Analytica Chimica Acta·T Alvarez-SeguraM C García-Alvarez-Coque
Aug 4, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Kazuki NakajimaYoshio Hirabayashi
Oct 25, 2016·Current Opinion in Biotechnology·Jennifer Haggarty, Karl Ev Burgess
Aug 22, 2017·Analytical Methods : Advancing Methods and Applications·Gabe NagyNicola L B Pohl
Aug 17, 2020·Journal of Chromatography. a·Shelby L WeatherbeeMaryanne M Collinson
May 12, 2021·Nature Methods·Leonardo Perez de SouzaAlisdair R Fernie
Jun 22, 2021·Journal of Chromatography. a·Szabolcs HorváthKrisztián Horváth