Serine protease-mediated host invasion by the parasitic nematode Steinernema carpocapsae.

The Journal of Biological Chemistry
Duarte ToubarroNelson Simoes

Abstract

Steinernema carpocapsae is an insect parasitic nematode used in biological control, which infects insects penetrating by mouth and anus and invading the hemocoelium through the midgut wall. Invasion has been described as a key factor in nematode virulence and suggested to be mediated by proteases. A serine protease cDNA from the parasitic stage was sequenced (sc-sp-1); the recombinant protein was produced in an Escherichia coli system, and a native protein was purified from the secreted products. Both proteins were confirmed by mass spectrometry to be encoded by the sc-sp-1 gene. Sc-SP-1 has a pI of 8.7, a molecular mass of 27.3 kDa, a catalytic efficiency of 22.2 × 10(4) s(-1) m(-1) against N-succinyl-Ala-Ala-Pro-Phe-pNA, and is inhibited by chymostatin (IC 0.07) and PMSF (IC 0.73). Sc-SP-1 belongs to the chymotrypsin family, based on sequence and biochemical analysis. Only the nematode parasitic stage expressed sc-sp-1. These nematodes in the midgut lumen, prepared to invade the insect hemocoelium, expressed higher levels than those already in the hemocoelium. Moreover, parasitic nematode sense insect peritrophic membrane and hemolymph more quickly than they do other tissues, which initiates sc-sp-1 expression. Ex vivo, Sc-SP...Continue Reading

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Citations

Oct 8, 2013·PloS One·Duarte ToubarroNelson Simões
Jun 16, 2014·Nature Genetics·Aaron R JexRobin B Gasser
Jan 8, 2015·Parasitology Research·Zhiyue LvHao Li
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Jun 12, 2019·Infection and Immunity·Shruti Yadav, Ioannis Eleftherianos
Oct 26, 2018·Parasites & Vectors·Muhammad MajeedMona Saleh
Oct 3, 2020·Microorganisms·Edit EszterbauerPavla Bartošová-Sojková
Jan 10, 2021·International Journal for Parasitology·Eric KenneyIoannis Eleftherianos

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