PMID: 9188613Jul 1, 1997Paper

Serine protease of pestiviruses: determination of cleavage sites

Journal of Virology
N TautzH-J Thiel

Abstract

The single-stranded genomic RNA of pestiviruses is of positive polarity and encompasses one large open reading frame of about 4,000 codons. The resulting polyprotein is processed co- and posttranslationally by virus-encoded and host cell proteases to give rise to the mature viral proteins. A serine protease residing in the nonstructural (NS) protein NS3 (p80) has been shown to be essential for the release of the NS proteins located downstream of NS3. In this report the NS3 serine protease-dependent cleavage sites for bovine viral diarrhea virus (BVDV) strain CP7 are described. Proteins used for analysis were generated in Escherichia coli or in eukaryotic cells by the use of the T7 vaccinia virus system. The N termini of NS4A, NS4B, NS5A, and NS5B were determined by protein sequencing. Analysis of the data obtained showed that leucine at P1 is the only position conserved for all cleavage sites. At P1' alanine is found at the NS4A-NS4B site, whereas serine resides at this position at the NS3-NS4A, NS4B-NS5A, and NS5A-NS5B cleavage sites. For all cleavage sites the amino acids found at P1 and P1' are conserved for different genotypes of pestiviruses, despite the high degree of sequence variation found between these viruses. It is ...Continue Reading

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Citations

Jan 23, 2010·The Journal of Biological Chemistry·Ioana Bintintan, Gregor Meyers
Jan 29, 2011·Journal of Virology·Benjamin LampTillmann Rümenapf
Jun 9, 2015·Animal Health Research Reviews·Martin BeerSandra Blome
Aug 25, 2017·Journal of Virology·Fengwei ZhengZishu Pan
May 28, 2003·Journal of Virology·Jin-Hua SunMin Gao
Sep 13, 2007·The Journal of Biological Chemistry·Birke Andrea Tews, Gregor Meyers
Feb 26, 2005·Journal of Virology·Young-Min LeeCharles M Rice
Apr 13, 2013·The Journal of General Virology·Peter Christian RisagerGraham J Belsham
Jun 28, 2012·Glycoconjugate Journal·Min FeiChanggeng Ruan
Aug 28, 2004·Journal of Virology·Israrul H AnsariRuben O Donis
Jul 28, 2010·The Journal of General Virology·Immanuel LeiferMartin Beer
Dec 1, 2011·BMC Research Notes·Immanuel LeiferNicolas Ruggli
Nov 16, 2016·Veterinary Record Open·C FactorF J Diéguez
Apr 22, 2017·Viruses·Sandra BlomeMartin Beer
Aug 30, 2017·FEBS Letters·Jian Lei, Rolf Hilgenfeld

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