Serine protease PRSS55 is crucial for male mouse fertility via affecting sperm migration and sperm-egg binding

Cellular and Molecular Life Sciences : CMLS
Xuan ShangZhugang Wang

Abstract

Testis-specific PRSS55 is a highly conserved chymotrypsin-like serine protease among mammalian species. So far, the physiological function of PRSS55 remains unknown. Here, we show that PRSS55 is a GPI-anchored membrane protein, specifically expressed in adult mouse testis and mainly observed in the luminal side of seminiferous tubules and sperm acrosome. Mice deficient for Prss55 develop male infertile with normal reproduction-related parameters observed. Interestingly, in vivo fertilization rate of Prss55-/- males is dramatically decreased, possibly due to incapable migration of Prss55-/- sperm from uterus into oviduct. However, in vitro fertilization rate has no difference between two genotypes although Prss55-/- sperm presents defective recognition/binding to zona-intact or zona-free oocytes. Further study reveals that mature ADAM3 is almost undetectable in Prss55-/- sperm, while precursor ADAM3 remains unchanged in the testis. However, it is shown that ADAM3 has no interaction with PRSS55 by immunoprecipitation with anti-PRSS55 antibody. The expression levels of several proteins known to be related to the observed phenotypes remain comparable between wt and Prss55-/- mice. Moreover, we found that Prss55 deficiency has no ef...Continue Reading

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Citations

May 28, 2019·Molecular Reproduction and Development·Wenfeng XiongChunling Shen
Feb 6, 2020·Journal of Cellular Physiology·Haihang ZhangFengchao Wang
Apr 18, 2020·Biology of Reproduction·Kiyonori KobayashiMasahito Ikawa
Jun 13, 2020·Biology of Reproduction·Tamara LarasatiMasahito Ikawa
Aug 8, 2020·Biology of Reproduction·Gagandeep Kaur Gahlay, Neha Rajput
Jul 10, 2020·Proceedings of the National Academy of Sciences of the United States of America·Surendra DawadiMartin M Matzuk
Apr 30, 2021·Development·Jason M ScovellCarolina J Jorgez
Jul 28, 2020·Molecular and Cellular Endocrinology·Marie Saint-DizierXavier Druart
Jan 23, 2022·Environmental Science and Pollution Research International·Fanli SunXuan Shang

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Datasets Mentioned

BETA
GSE107350

Methods Mentioned

BETA
contraception
genotyping
electrophoresis
PCR
protein assay
transfection

Software Mentioned

- Spring
Gene

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