Serine-threonine protein kinase activation may be an effective target for reducing neuronal apoptosis after spinal cord injury

Neural Regeneration Research
Mu JinShi-Yao Liu

Abstract

The signaling mechanisms underlying ischemia-induced nerve cell apoptosis are poorly understood. We investigated the effects of apoptosis-related signal transduction pathways following ischemic spinal cord injury, including extracellular signal-regulated kinase (ERK), serine-threonine protein kinase (Akt) and c-Jun N-terminal kinase (JNK) signaling pathways. We established a rat model of acute spinal cord injury by inserting a catheter balloon in the left subclavian artery for 25 minutes. Rat models exhibited notable hindlimb dysfunction. Apoptotic cells were abundant in the anterior horn and central canal of the spinal cord. The number of apoptotic neurons was highest 48 hours post injury. The expression of phosphorylated Akt (p-Akt) and phosphorylated ERK (p-ERK) increased immediately after reperfusion, peaked at 4 hours (p-Akt) or 2 hours (p-ERK), decreased at 12 hours, and then increased at 24 hours. Phosphorylated JNK expression reduced after reperfusion, increased at 12 hours to near normal levels, and then showed a downward trend at 24 hours. Pearson linear correlation analysis also demonstrated that the number of apoptotic cells negatively correlated with p-Akt expression. These findings suggest that activation of Akt m...Continue Reading

References

Nov 24, 1995·Science·Z XiaM E Greenberg
Feb 1, 1995·Journal of Neurotrauma·D M BassoJ C Bresnahan
Dec 12, 1997·Biochimica Et Biophysica Acta·A Minden, M Karin
Apr 30, 1998·Current Opinion in Cell Biology·Y T Ip, R J Davis
Mar 10, 2001·Nature·L Chang, M Karin
Apr 27, 2001·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·S YanoY Ushio
Oct 13, 2001·Brain Research. Molecular Brain Research·D A Shackelford, R Y Yeh
Dec 12, 2001·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·N NoshitaP H Chan
May 9, 2002·The Annals of Thoracic Surgery·Joseph S CoselliZachary C Schmittling
Jul 25, 2003·Brain Research. Molecular Brain Research·Deborah A Shackelford, Richard Y Yeh
May 18, 2005·Neurobiology of Disease·Fengshan YuPak H Chan
Oct 7, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ertugrul KilicDirk M Hermann
May 17, 2006·Biochemical and Biophysical Research Communications·Bahareh BadrianMarie A Bogoyevitch
Jun 28, 2006·The Journal of Pharmacology and Experimental Therapeutics·Shougang Zhuang, Rick G Schnellmann
Oct 13, 2006·Neuroscience Letters·Jeong-Sun ChoiMun-Yong Lee
May 25, 2012·Acta Anaesthesiologica Scandinavica·Y W YangW P Cheng

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis