PMID: 10564085Nov 24, 1999Paper

Serine/threonine protein phosphatases and regulation of K-Cl cotransport in human erythrocytes

The American Journal of Physiology
I BizeC Brugnara

Abstract

Activation of K-Cl cotransport is associated with activation of membrane-bound serine/threonine protein phosphatases (S/T-PPases). We characterize red blood cell S/T-PPases and K-Cl cotransport activity regarding protein phosphatase inhibitors and response to changes in ionic strength and cell size. Protein phosphatase type 1 (PP1) activity is highly sensitive to calyculin A (CalA) but not to okadaic acid (OA). PP2A activity is highly sensitive to CalA and OA. CalA completely inhibits K-Cl cotransport activity, whereas OA partially inhibits K-Cl cotransport. Membrane PP1 and membrane PP2A activities are elevated in cells suspended in hypotonic solutions, where K-Cl cotransport is elevated. Increases in membrane PP1 activity (62 +/- 10% per 100 meq/l) result from decreases in intracellular ionic strength and correlate with increases in K-Cl cotransport activity (54 +/- 10% per 100 meq/l). Increases in membrane PP2A activity (270 +/- 77% per 100 mosM) result from volume increases and also correlate with increases in K-Cl cotransport activity (420 +/- 47% per 100 mosM). The characteristics of membrane-associated PP1 and PP2A are consistent with a role for both phosphatases in K-Cl cotransport activation in human erythrocytes.

References

Nov 1, 1992·The American Journal of Physiology·P K LaufE Delpire
Oct 1, 1991·The American Journal of Physiology·E P OrringerJ C Parker
Jan 1, 1991·The American Journal of Physiology·D M Kaji, Y Tsukitani
Mar 31, 1989·Biochemical and Biophysical Research Communications·H IshiharaD Uemura
Jan 1, 1989·Annual Review of Biochemistry·P Cohen
Feb 1, 1988·The American Journal of Physiology·S Grinstein, W Furuya
Mar 1, 1987·The American Journal of Physiology·C Brugnara, D C Tosteson
Oct 1, 1995·The American Journal of Physiology·I Bize, P B Dunham
Jan 1, 1995·International Review of Cytology·E K Hoffmann, P B Dunham
Nov 9, 1995·The New England Journal of Medicine·M L McManusK Strange
Oct 1, 1995·The Biochemical Journal·S Wera, B A Hemmings
May 14, 1993·Biochemical and Biophysical Research Communications·H ShimaM Nagao
Jan 1, 1995·Pflügers Archiv : European journal of physiology·J S Gibson, A C Hall
Jul 21, 1994·Biochimica Et Biophysica Acta·A K LarsenE K Hoffmann
Mar 1, 1994·The American Journal of Physiology·I Bize, P B Dunham
Jan 1, 1993·The American Journal of Physiology·L C Starke, M L Jennings
Mar 1, 1996·The American Journal of Physiology·T Krarup, P B Dunham
Jul 1, 1996·The American Journal of Physiology·P W FlatmanP K Lauf
Aug 1, 1996·Pflügers Archiv : European journal of physiology·Y R Weaver, A R Cossins
Feb 1, 1996·The American Journal of Physiology·A N Namboodiripad, M L Jennings
Oct 1, 1996·The American Journal of Physiology·O Ortiz-CarranzaP K Lauf
Jan 15, 1997·The Journal of Clinical Investigation·L De FranceschiG Berton
Jul 1, 1997·Trends in Biochemical Sciences·P T Cohen
Nov 18, 1997·Clinical and Experimental Pharmacology & Physiology·N S al-Rohil Gharaibeh, M al-Sheyyab
Apr 16, 1998·Pflügers Archiv : European journal of physiology·S J CullifordP F Speake

❮ Previous
Next ❯

Citations

May 10, 2003·Pflügers Archiv : European journal of physiology·P MercirisF Giraud
Oct 6, 2009·American Journal of Hematology·Emmanuelle Varlet-MarieDavid Piquemal
Oct 30, 2015·Journal of Neurophysiology·Kristopher T Kahle, Eric Delpire
Nov 16, 2019·Pflügers Archiv : European journal of physiology·David C-Y LuJohn S Gibson
Jul 31, 2004·Acta Physiologica Scandinavica·M SalomonssonN-H Holstein-Rathlou
Feb 28, 2003·American Journal of Physiology. Cell Physiology·Isabel BizeCarlo Brugnara
Dec 30, 2008·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Natalia Ivanovna Agalakova, G P Gusev
Nov 26, 2009·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Gennadii Petrovich Gusev, Natalia Ivanovna Agalakova
Dec 25, 2004·Physiological Reviews·Virgilio L Lew, Robert M Bookchin
Nov 12, 2005·Pflügers Archiv : European journal of physiology·Lucia De FranceschiGiorgio Berton
Feb 16, 2005·The Journal of Membrane Biology·N C AdragnaP K Lauf
Mar 25, 2005·The European Journal of Neuroscience·Stanislav KhirugLeonard Khiroug
Mar 10, 2017·Journal of Cellular Physiology·Rachelle Frenette-CottonPaul Isenring
Apr 5, 2018·Clinical Hemorheology and Microcirculation·Carlo Brugnara
Apr 6, 2006·The Journal of Biological Chemistry·Marc J BergeronPaul Isenring
Jul 27, 2000·The Journal of Biological Chemistry·A MercadoG Gamba
Feb 5, 2002·Annual Review of Physiology·Eric Delpire, David B Mount
Jan 8, 2009·Physiological Reviews·Else K HoffmannStine F Pedersen
Feb 12, 2009·Journal of Cellular Physiology·Marc J BergeronPaul Isenring
Aug 9, 2012·The Biochemical Journal·Adam WieschhausAthar H Chishti
Aug 16, 2000·American Journal of Physiology. Cell Physiology·K StrangeE Delpire
Aug 10, 2001·Current Opinion in Nephrology and Hypertension·D B Mount, G Gamba
Jul 29, 2005·American Journal of Physiology. Renal Physiology·Adriana MercadoDavid B Mount
Dec 14, 2019·Blood Cells, Molecules & Diseases·Boris E ShmuklerSeth L Alper
Sep 30, 2003·Journal of the American Society of Nephrology : JASN·Pernille B HansenJurgen Schnermann
May 19, 2001·Blood Cells, Molecules & Diseases·C Brugnara
Sep 27, 2000·Biochimica Et Biophysica Acta·B E ShmuklerS L Alper
Oct 25, 2001·Journal of the American Society of Nephrology : JASN·Bert FlemmingPontus B Persson
Sep 25, 2002·Journal of Theoretical Biology·C FåhraeusW Grampp
Mar 25, 2004·Bioelectrochemistry·Erwin WeissIngolf Bernhardt
Aug 26, 2004·Biochimica Et Biophysica Acta·H EbelT Günther
Dec 15, 2005·Zoology : Analysis of Complex Systems, ZACS·D Kültz
Oct 24, 2007·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Norma C Adragna, Peter K Lauf
Nov 6, 2012·Blood Cells, Molecules & Diseases·Alicia RiveraSoohee Lee

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