PMID: 3320514Oct 1, 1987Paper

Serodiagnosis of cilia-associated respiratory bacillus infection by the indirect immunofluorescence assay technique

Laboratory Animals
S MatsushitaH Joshima

Abstract

Antibody to cilia-associated respiratory (CAR) bacillus was detected by the indirect immunofluorescence assay (IFA) technique using tracheal sections of infected mice as antigen in serum samples collected from rats infected naturally and experimentally. Nine of 23 cases of natural infection were positive in IFA antibody, with titres ranging from 1:10 to 1:80, and all these antibody-positive cases were also histologically positive. The remaining 14 cases were negative in both IFA antibody and histological diagnosis, even though some of them were infected with Sendai virus and Mycoplasma pulmonis. In the experimental infection, serum samples collected from 18 rats on days 4, 7, 14, 21, 28 and 56 post-inoculation (PI) (three rats for each point) and examined for IFA antibody revealed that seroconversion occurred in one rat on day 14 PI and in three rats on day 21 PI. Antibody titres of 1:80 to 1:160 remained to the termination of the experiment. The IFA technique was useful for the diagnosis of CAR bacillus infection except in the early stage of the infection.

Citations

Aug 28, 1998·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·M CaniattiE Scanziani
Dec 24, 2003·Comparative Immunology, Microbiology and Infectious Diseases·H Takahashi-OmoeK Yamamoto
May 19, 2009·Experimental Animals·Toshiaki Kokubo, Satoru Matsushita
Aug 17, 2000·The Journal of Veterinary Medical Science·A KawanoK Mita
Jan 1, 1993·Veterinary Pathology·A T HastieA M Allen
Jun 10, 2021·PloS One·Martina NačeradskáRichard Malik

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