Serodiagnosis of respiratory synctial virus infections in infants and young children by the immunoperoxidse technique.

Journal of Clinical Microbiology
G GernaG Achilli

Abstract

The immunoperoxidase antibody (IPA) technique was used to develop two new tests for serodiagnosis of respiratory syncytial virus infections: a microneutralization test based on the reduction of the number of infected cells and an IPA test for determination of virus-specific immunoglobulin G (IgG). Neutralizing antibody was determined both in the presence and absence of complement. In a group of 24 infants and young childres, ages less than 1 to 36 months, with acute respiratory syncytial virus infection, serodiagnosis was made by the IPA-IgG test in 20 cases, by neutralization test with addition of complement in 19 cases, and by neutralization test without addition of complement in 17 cases. Complement fixation detected only 12 cases of infection. All four cases not serologically diagnosed were infants less than 1 month old. Neutralization test antibody titers in the presence of complement were usually 4- to 16-fold higher than titers obtained without addition of complement. Both IPA-IgG and neutralization test (in the presence of complement) appear very efficient in serologically detecting respiratory syncytial virus infections in infants older than 1 month and give rapid results (IPA-IgG takes 2 h to complete, and the neutral...Continue Reading

References

Mar 1, 1976·Journal of Clinical Microbiology·G GernaR W Chambers
Feb 1, 1979·Proceedings of the Society for Experimental Biology and Medicine·E B BuynakM R Hilleman
Oct 1, 1972·Journal of Clinical Pathology·G E Urquhart, G H Walker
Oct 1, 1971·Archives of Disease in Childhood·C A RossF A Assaad
Oct 1, 1965·Proceedings of the Society for Experimental Biology and Medicine·J H SchiebleA Kase
Mar 13, 1964·Archiv für die gesamte Virusforschung·C A ROSSI A CROWTHER

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