Serodiagnosis, targeting nonstructural protein 4, of porcine reproductive and respiratory syndrome virus

Archives of Virology
Shengliang CaoYihong Xiao

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) causes great economic losses to the swine industry worldwide. Typically, an N protein-coated indirect enzyme-linked immunosorbent assay (N-coated iELISA) is used to detect PRRSV antibodies. Non-structural protein (NSP) 4 is essential to the PRRSV life cycle and contains B-cell epitopes. Yet, no specific antibody against NSP4 has been detected in clinical samples. In this study, we developed an NSP4-coated iELISA and compared its effectiveness with the N-coated iELISA. The NSP4-coated iELISA was developed with a cut-off value of 0.406 at an optical density of 450 nm by testing a panel of 70 PRRSV positive and 80 PRRSV negative pig serum samples, which generated a specificity and sensitivity of 100%. Agreement between the NSP4-coated and N-coated iELISAs was 92.2%. Interestingly, 50 serum samples, mostly from pigs vaccinated with the HP-PRRSV live strain, tested positive for PRRSV antibodies with the NSP4-coated iELISA, but were negative with the N-coated iELISA. These results were further confirmed by western blot analysis and another iELISA based on the N-terminus of NSP2 (NSP2-1-coated iELISA). The agreement between the results of western blot analysis with the NSP4-c...Continue Reading

References

Mar 22, 2007·The Journal of General Virology·Craig R JohnsonMichael P Murtaugh
Feb 7, 2008·Emerging Infectious Diseases·Guang-Zhi TongHua-Ji Qiu
Nov 20, 2015·Emerging Infectious Diseases·Lei ZhouHanchun Yang
Dec 10, 2015·Annual Review of Animal Biosciences·Joan K LunneyGourapura J Renukaradhya
Jun 12, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Minxia ZhangYihong Xiao

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
iELISA
PCR
electrophoresis
ELISA
iELISAs

Software Mentioned

GraphPad Prism
TG
ROC
graph (
- ROC )

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