Serodiagnostic comparison between two methods, ELISA and surface plasmon resonance for the detection of antibody titres of Mycoplasma hyopneumoniae

Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health
T J KimJ I Lee

Abstract

A protein chip based on surface plasmon resonance (SPR) was developed for measuring the Mycoplasma hyopneumoniae antibody titres using a recombinant 30-kDa fragment of P97 adhesin as an antigen. The diagnostic potential of this SPR assay, for detecting the antibody titres to the M. hyopneumoniae 30-kDa protein, was compared with that of conventional ELISA using 70 pig serum samples taken from six pig farms. The SPR assay was found to be highly specific and sensitive. Moreover, there was a strong positive correlation between the SPR and ELISA titres (n = 70, r = 0.898, P < 0.01). Therefore, this recombinant 30-kDa protein can be used as an antigen for serological studies, and the SPR, which is a label-free method, is expected to be a valuable and reproducible tool in the serodiagnosis of M. hyopneumoniae infection.

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Citations

Mar 20, 2001·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·E Rautiainen, P Wallgren
Sep 23, 2008·Journal of Microbiological Methods·Saikat Datta MazumdarMichael Keusgen
Aug 21, 2013·Journal of Pharmaceutical and Biomedical Analysis·Theo RispensDesiree van der Kleij
Jul 5, 2007·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Ho Seong Cho, Tae Jung Kim
Jun 28, 2007·Yonsei Medical Journal·Sang-Nae Cho
Dec 13, 2007·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Dec 1, 2009·Applied Magnetic Resonance·I SepkhanovaM Huber
Jul 2, 2020·Diagnostics·Dina M Alhaj-QasemFatin Hamimi Mustafa

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