Serogroup conversion of Vibrio cholerae

Canadian Journal of Microbiology
R R ColwellB Xu

Abstract

Vibrio cholerae serogroup O1 can be detected in the environment in a viable but nonculturable form, whereas V. cholerae non-O1 cells can be readily cultured during interepidemic periods in geographical regions where cholera is endemic. In the present study, pure cultures of V. cholerae non-O1 cells contained O1 cells when examined by immune-fluorescence microscopy. Laboratory microcosms were used to examine the outgrowth of the O1 cells in cultures of non-O1 V. cholerae. One O1 cell per 10(6) non-O1 cells could be detected by direct fluorescent-monoclonal antibody staining but only after incubation of the non-O1 culture for 48 h. Individual O1 cells were not detected in cultures incubated less than 48 h. Hybridization study, using a polymerase chain reaction (PCR) amplified fragment of the O-antigen of V. cholerae O1 as a probe, revealed the existence of a homologous gene in a microcosm sample of V. cholerae non-O1 containing serogroup-converted cells. The mechanism by which O1 cells can occur in cultures of non-O1 V. cholerae most likely resulted from spontaneous mutation of gene(s) encoding the O-somatic properties and (or) chemical, physical, or biological changes in the environment inducing expression or repression of the c...Continue Reading

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Citations

Feb 1, 1994·Research in Microbiology·A CoelhoC A Salles
Feb 9, 2008·Transactions of the Royal Society of Tropical Medicine and Hygiene·Nilma Cintra LealErnesto Hofer
May 12, 2010·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Hemant Kumar KhuntiaBibhuti Bhusan Pal
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Sep 28, 2021·Frontiers in Microbiology·Imchang LeeJongsik Chun

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