Serologic and biochemical analysis of latent a1 IgG

Molecular Immunology
M Abolhassani, K H Roux

Abstract

Immunization of rabbits with anti-allotype antibody (Ab) induces at least two populations of highly cross-reactive molecules. One of these populations bears the nominal VHa allotype of the producing rabbit and is designated the VHa-positive anti-IdX Ab. The other population lacks the expected nominal allotype and thus could represent an induced latent allotype-bearing Ig. To define better the putative latent allotypes, they were subjected to serologic, electron microscopic and biochemical analyses. The induced latent-like population was compared to nominal a1 and found to be indistinguishable in inhibition assays incorporating both rabbit anti-a1 Ab and mouse monoclonal anti-a1 Ab. In contrast, the latent-like Ig was less inhibitory than normal a1 Ig in assays with goat and guinea-pig anti-a1 Ab. The isolated anti-IdX population was less inhibitory than either nominal or latent a1 Ig in all assays. Immunoelectron microscopic analysis indicates that complexes composed of latent-like a1 molecules and Fab anti-a1 ab resemble allotype/anti-allotype (i.e. a1/anti-a1) complexes. Tryptic digests of the putative latent a1 H-chains reveals that these molecules share an a1-specific peptide with digests of nominal a1 H-chain. The peptides...Continue Reading

References

Mar 15, 1978·Biochemical and Biophysical Research Communications·D N Podell, G N Abraham
Jun 1, 1971·Analytical Chemistry·M Roth
Jun 1, 1981·European Journal of Immunology·J Roland, P A Cazenave
Jan 1, 1983·Annals of the New York Academy of Sciences·W J Horng, D S Kazdin

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