Serologic diagnosis of canine and equine borreliosis: use of recombinant antigens in enzyme-linked immunosorbent assays.

Journal of Clinical Microbiology
L MagnarelliE Fikrig

Abstract

Serum samples from dogs and equids suspected of having canine or equine borreliosis, respectively, were analyzed in polyvalent enzyme-linked immunosorbent assays (ELISAs) with whole-cell or recombinant antigens of Borrelia burgdorferi sensu stricto. Purified preparations of recombinant antigens included outer surface protein A (OspA), OspB, OspC, OspE, OspF, and p41-G (a fragment of flagellin). Of the 36 dog sera that reacted positively to whole-cell antigen, 32 (88.9%) contained antibodies to one or more recombinant antigens. Reactivities to OspF (88.9% positive) and p41-G (75% positive) were most prevalent. In analyses of 30 equid sera positive in an ELISA with whole cells, 24 (80%) contained antibodies to one or more recombinant antigens. Seropositivities in ELISAs with p41-G (50% positive) and OspF (46.7% positive) were more than twofold greater than in ELISAs with OspA, OspB, or OspC (10 to 20% positive). In parallel tests of eight canine and three equine sera, there was good agreement in results of Western blot (immunoblot) analyses and ELISAs. Although dog and equid sera with antibodies to whole-cell B. burgdorferi frequently reacted positively to one or more recombinant antigens, the inclusion of OspF and p41-G antigens...Continue Reading

References

Aug 31, 1989·The New England Journal of Medicine·A C Steere
Mar 1, 1995·The Journal of Infectious Diseases·M A GerberS J Padula
Jul 1, 1994·Journal of Clinical Microbiology·S J PadulaR W Ryan
Jul 1, 1994·The Journal of Clinical Investigation·S E SchutzerM Brunner
Jan 1, 1994·Infection and Immunity·T T LamR A Flavell
Nov 1, 1993·Journal of Clinical Microbiology·L A Magnarelli, J F Anderson
Jan 1, 1996·The Journal of Experimental Medicine·A M de SilvaE Fikrig
Feb 1, 1996·Journal of Clinical Microbiology·L A MagnarelliR A Flavell

❮ Previous
Next ❯

Citations

Jun 3, 1999·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·D T Gauthier, L S Mansfield
Dec 1, 2002·The Veterinary Quarterly·C M ButlerJ H van der Kolk
Apr 4, 2014·Clinical and Vaccine Immunology : CVI·Elisabeth BaumAlan G Barbour
Feb 18, 2012·Clinical and Vaccine Immunology : CVI·Bettina WagnerPatrick Meeus
Aug 23, 2008·Journal of Medical Microbiology·Louis A MagnarelliRaymond A Koski
Oct 6, 2000·Journal of the American Veterinary Medical Association·L A MagnarelliE Fikrig
Feb 23, 2018·Journal of Veterinary Internal Medicine·T J DiversA L Johnson
Oct 17, 2012·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Fabíola do Nascimento CorrêaAdivaldo Henrique da Fonseca
Jul 6, 2000·Journal of Clinical Microbiology·M A GuerraU Kitron

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.