Serologic distinction between the rabbit kappa L chain allotype b4 and an allele b4

European Journal of Immunology
L J SmithW J Mandy

Abstract

Rabbit kappa light (L) chain allotypes are controlled by the allelic genes b4, b5, b6, b9 and a recently described allele b4v. The product of the b4v gene differs from that of b4 by at least two amino acid substitutions in the constant region. A serologic distinction between the b4 and b4v allotyes has now been detected by a radioimmune assay using one of six anti-b4 sera tested. The distinction was enhanced by fractionation of this antiserum on an immunoadsorbent containing cottontail rabbit IgG. IgG with L chains of the b4v type are deficient in their ability to inhibit binding of b4 IgG to the antibody preparation. L chains isolated from b4 and b4v IgG samples could not be distinguished by this assay indicating that interaction with an H chain is required for expression of the L chain determinants that differentiate b4 from b4v.

References

Jan 1, 1975·Advances in Immunology·T J Kindt
Jul 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E A KabatD R Davies
Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·V FarnsworthL Hood
Jun 1, 1976·The Journal of Experimental Medicine·J A Sogn, T J Kindt
May 1, 1976·Immunochemistry·J Teherani, W J Mandy
May 13, 1967·Nature·S Dubiski, P J Muller
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·K C ChenR M Krause
Nov 1, 1970·Immunochemistry·S Sonoda, M Schlamowitz
Jan 1, 1960·Proceedings of the Society for Experimental Biology and Medicine·H B LEVY, H A SOBER
Sep 1, 1940·The Biochemical Journal·R A Kekwick

Related Concepts

Kappa allotype b4, rabbits
Alleles
Antibody Specificity
Binding Sites, Antibody
Immune Sera
Immunoglobulin Allotypes
Kappa-Immunoglobulin Subgroup VK-21
Immunoglobulin Light Chains
Genealogical Tree

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.