Serological analysis of Ebola virus survivors and close contacts in Sierra Leone: A cross-sectional study

PLoS Neglected Tropical Diseases
Peter J HalfmannFoday Sahr

Abstract

The 2013-2016 Ebola virus outbreak in West Africa was the largest and deadliest outbreak to date. Here we conducted a serological study to examine the antibody levels in survivors and the seroconversion in close contacts who took care of Ebola-infected individuals, but did not develop symptoms of Ebola virus disease. In March 2017, we collected blood samples from 481 individuals in Makeni, Sierra Leone: 214 survivors and 267 close contacts. Using commercial, quantitative ELISAs, we tested the plasma for IgG-specific antibodies against three major viral antigens: GP, the only viral glycoprotein expressed on the virus surface; NP, the most abundant viral protein; and VP40, a major structural protein of Zaire ebolavirus. We also determined neutralizing antibody titers. In the cohort of Ebola survivors, 97.7% of samples (209/214) had measurable antibody levels against GP, NP, and/or VP40. Of these positive samples, all but one had measurable neutralizing antibody titers against Ebola virus. For the close contacts, up to 12.7% (34/267) may have experienced a subclinical virus infection as indicated by detectable antibodies against GP. Further investigation is warranted to determine whether these close contacts truly experienced subc...Continue Reading

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Citations

Jun 8, 2021·Frontiers in Immunology·Dominic Paquin-ProulxMichael A Eller
Feb 24, 2021·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Mamadou Saliou Kalifa DialloEric Delaporte
Sep 1, 2021·Current Opinion in Virology·Asmaa HachimSophie A Valkenburg

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Methods Mentioned

BETA
ELISA
ELISAs

Software Mentioned

GraphPad Prism

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