Serological Analysis of New York City COVID19 Convalescent Plasma Donors.

MedRxiv : the Preprint Server for Health Sciences
Larry L LuchsingerTheodora Hatziioannou


The development of neutralizing antibodies (NAbs) against SARS-CoV-2, following infection or vaccination, is likely to be critical for the development of sufficient population immunity to drive cessation of the COVID19 pandemic. A large number of serologic tests, platforms and methodologies are being employed to determine seroprevalence in populations to select convalescent plasmas for therapeutic trials, and to guide policies about reopening. However, these tests have substantially variable sensitivity and specificity, and their ability to quantitatively predict levels of NAbs is unknown. We determined levels of antibodies in convalescent plasma using commercially available SARS-CoV-2 detection tests and in-house ELISA assays and correlated those measurements with neutralization activity measured using pseudotyped virus particles, which offer the most informative assessment of antiviral activity of patient sera against viral infection. Our data show that a large proportion of convalescent plasma samples have modest antibody levels and that commercially available tests have varying degrees of accuracy in predicting neutralizing activity. Nevertheless, we found particular commercially available tests are capable of accurately me...Continue Reading


Jun 28, 2020·Journal of Virology·John P Moore, P J Klasse
Aug 19, 2020·Nature Medicine·Adam G LaingAdrian C Hayday
Oct 8, 2020·Signal Transduction and Targeted Therapy·Hao ZengQuanming Zou
Jan 26, 2021·Transfusion·Alfredo Mendrone-JuniorEster Cerdeira Sabino
Oct 7, 2020·Journal of Clinical Microbiology·Elitza S TheelAudrey N Schuetz

❮ Previous
Next ❯

Methods Mentioned

enzyme linked immunosorbent assays

Related Concepts

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.