Serological Analysis of New York City COVID19 Convalescent Plasma Donors.

MedRxiv : the Preprint Server for Health Sciences
Larry L LuchsingerTheodora Hatziioannou

Abstract

The development of neutralizing antibodies (NAbs) against SARS-CoV-2, following infection or vaccination, is likely to be critical for the development of sufficient population immunity to drive cessation of the COVID19 pandemic. A large number of serologic tests, platforms and methodologies are being employed to determine seroprevalence in populations to select convalescent plasmas for therapeutic trials, and to guide policies about reopening. However, these tests have substantially variable sensitivity and specificity, and their ability to quantitatively predict levels of NAbs is unknown. We determined levels of antibodies in convalescent plasma using commercially available SARS-CoV-2 detection tests and in-house ELISA assays and correlated those measurements with neutralization activity measured using pseudotyped virus particles, which offer the most informative assessment of antiviral activity of patient sera against viral infection. Our data show that a large proportion of convalescent plasma samples have modest antibody levels and that commercially available tests have varying degrees of accuracy in predicting neutralizing activity. Nevertheless, we found particular commercially available tests are capable of accurately me...Continue Reading

Citations

Jun 28, 2020·Journal of Virology·John P Moore, P J Klasse
Aug 19, 2020·Nature Medicine·Adam G LaingAdrian C Hayday
Oct 8, 2020·Signal Transduction and Targeted Therapy·Hao ZengQuanming Zou
Jan 26, 2021·Transfusion·Alfredo Mendrone-JuniorEster Cerdeira Sabino
Oct 7, 2020·Journal of Clinical Microbiology·Elitza S TheelAudrey N Schuetz

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Methods Mentioned

BETA
enzyme linked immunosorbent assays
ELISA
PCR
ELISAs

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