Serological and biological activities of anti-Haemophilus influenzae ribosomal serum.

Infection and Immunity
M A KatzM Solotorovsky

Abstract

The antibody content in serum from rabbits immunized with ribosomes from Haemophilus influenzae type b was determined by passive hemagglutination, enzyme-linked immunosorbent assay, and complement fixation. Attempts to use passive hemagglutination to assay anti-ribosomal antibodies were unsuccessful. In the enzyme-linked immunosorbent assay tests, rabbit antiserum was allowed to react with ribosomes that adhered to microtiter plates. The enzyme-linked immunosorbent assay method detected, in two ribosome-immunized rabbits and by 3 days postimmunization, titers which rose to plateaus on days 24 to 31 and declined thereafter. With the complement fixation method, the serum from one immunized rabbit also showed a titer on day 3 and reached a plateau on days 20 to 31. Serum from the other immunized rabbit did not develop a titer until day 11; it reached a lower plateau on days 20 to 24 and then declined on days 27 to 55. Although there were no apparent differences between the two immunized rabbits by the enzyme-linked immunosorbent assay, there were differences between the complement fixation antibodies in these rabbits. Passive protection experiments were performed with these sera. Maximal passive protection was achieved when mice w...Continue Reading

References

Feb 1, 1977·Infection and Immunity·M LynnM Solotorovsky
Dec 17, 1971·Biochemical and Biophysical Research Communications·P D Weston, S Avrameas
Jun 24, 1971·FEBS Letters·B K. Van Weemen, A H.W.M. Schuurs

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