Serological assays to discriminate rabbit haemorrhagic disease virus from Australian non-pathogenic rabbit calicivirus

Veterinary Microbiology
June LiuTanja Strive

Abstract

Serological cross reactivity between the virulent rabbit haemorrhagic disease virus (RHDV) and the closely related but non-pathogenic rabbit calicivirus (RCV) makes it difficult to study the epidemiology of each virus and the interaction between them when both viruses co-circulate in wild rabbit populations. ELISA methods for the diagnosis of RHDV infection are well characterized, but no specific serological tests for RCV have been developed. Following the characterization of Australian non-pathogenic RCV-A1 strains, we used virus-like-particles (VLPs) and anti-RCV-A1 specific antibodies to establish a set of isotype ELISAs for detection of IgG, IgA and IgM in rabbit sera and secretory mucosal IgA in rectal swabs, and two competition ELISAs. These assays were used to discriminate between anti-RCV-A1 and anti-RHDV antibodies in rabbits. The isotype ELISAs were highly sensitive for detection of anti-RCV-A1 antibodies, but varying levels of cross reactivity from anti-RHDV antibodies occurred in the isotype ELISAs and one competition ELISA. However, the second competition ELISA specifically detected antibodies to RCV-A1 and showed no cross reactivity to anti-RHDV sera. These ELISAs provide important tools to monitor RCV-A1 infectio...Continue Reading

References

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Citations

May 7, 2013·The Veterinary Clinics of North America. Exotic Animal Practice·Peter J Kerr, Thomas M Donnelly
Jan 2, 2015·Evolutionary Applications·Nina I SchwensowSimone Sommer
Apr 2, 2017·Applied and Environmental Microbiology·Leila J NicholsonJanine A Duckworth
Dec 21, 2017·Emerging Infectious Diseases·Jackie E MaharRobyn N Hall
Aug 23, 2018·The Journal of Animal Ecology·Konstans WellsNina I Schwensow

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