PMID: 6535640Dec 1, 1984Paper

Serological characterization of a pluripotent mouse embryonal stem cell line, two transformed derivatives, and an endoderm-like cell line

Cell Differentiation
G WalterJ Schöneich

Abstract

The pluripotent mouse embryonal stem cell line BLC 1 and two transformants derived from it by DNA transformation (T1 and T2/K26) as well as the blastocyst-derived cell line BLC 3 were tested for the presence of cell surface antigens recognized by the monoclonal antibodies ECMA-7, anti-SSEA-1 and M 1/22.25, and intermediate filament proteins labeled by the monoclonal antibodies TROMA 1 and TROMA 2 using a three-step indirect immunofluorescence technique. According to present concepts and in agreement with previous data (Wobus et al., 1984a), the results obtained indicate that BLC 1, T1 and T2/K26 are undifferentiated embryonal stem cells, and BLC 3 is an endoderm-like cell line.

References

Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D Solter, B B Knowles
Nov 6, 1975·Nature·V E PapaioannouM J Evans
May 1, 1984·Experimental Cell Research·A M WobusJ Schöneich
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T A Stewart, B Mintz
Jan 1, 1980·Journal of Immunological Methods·B Uchańska-ZieglerA Ziegler

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Citations

Nov 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·O RoseA Starzinski-Powitz
Sep 13, 2005·In Vitro Cellular & Developmental Biology. Animal·Neil C TalbotWesley M Garrett
Jun 16, 2007·In Vitro Cellular & Developmental Biology. Animal·Neil C TalbotWesley M Garrett
Jan 1, 1991·Mammalian Genome : Official Journal of the International Mammalian Genome Society·J M FriedmanN Bahary
Jan 1, 1994·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Anna M WobusJürgen Hescheler

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