Serological comparison of systemic lupus erythematosus with neuropsychiatric lupus using synthetic nucleic acid antigens

Journal of Translational Autoimmunity
Sangita KhatriKira Astakhova

Abstract

Neuropsychiatric systemic lupus erythematosus is an autoimmune disorder characterized by an irregular exchange between the central nervous system and the immune system, leading to the outbreak of neurological conditions with possible disabling effects. Although neuropsychiatric systemic lupus erythematosus is the most common expression of lupus condition, it is still poorly understood. In this study, we focus on the development of an advantageous method based on the application of synthetic nucleic acids and protein-based antigen arrays in order to characterize autoreactive antibodies in neuropsychiatric systemic lupus erythematosus. We confirmed the benefits of using synthetic oligonucleotides such as assay reproducibility, elevated affinity and specificity to autoreactive antibodies. We also demonstrated presence of autoantibodies towards three particular synthetic double stranded antigens and verify similarity of antinuclear antibody patterns in ordinary lupus and neuropsychiatric systemic lupus erythematosus.

References

May 29, 2000·Applied Biochemistry and Biotechnology·L S RodkeyE W Voss
Feb 9, 2002·Biotechnology and Applied Biochemistry·Geert Besselink, Dirk de Korte
Jun 4, 2002·Best Practice & Research. Clinical Rheumatology·David Hermosillo-Romo, Robin L Brey
Sep 18, 2013·Artificial DNA, PNA & XNA·T Santhosh KumarIrina Kira Astakhova
Feb 12, 2014·Nature Reviews. Rheumatology·John G Hanly
Nov 20, 2015·Nature Reviews. Rheumatology·David S Pisetsky
Jun 15, 2017·Chembiochem : a European Journal of Chemical Biology·Maria TaskovaKira Astakhova
Apr 5, 2018·Scientific Reports·Martin KleckaKira Astakhova
Jun 22, 2018·Arthritis & Rheumatology·John G HanlyJulius Birnbaum

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
antigen array
ELISA

Software Mentioned

R package mcglm

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

© 2021 Meta ULC. All rights reserved