Serological cross reactivity between polyomavirus capsids

Advances in Experimental Medicine and Biology
Raphael Viscidi, Barbara Clayman

Abstract

Multiple methods have been used to measure antibodies to polyomavirus virions. In order to have a common method for all polyomaviruses, we developed enzyme immunoassays (EIAs) using virus-like-particles (VLPs) produced in the baculovirus expression system. We tested serum samples from humans and rhesus macaques in VLP-based EIAs for the two human polyomaviruses, BK and JC virus, and two nonhuman primate polyomaviruses, simian virus 40 (SV40) and lymphotropic polyomavirus (LPV). Rhesus sera exhibited low level reactivity to BK and JC, and approximately 10 and 15% of human sera showed low level reactivity to SV40 and LPV, respectively. Competitive inhibition assays with VLP protein demonstrated that the reactivity of rhesus sera against BK and JC VLPs was blocked by both SV40 and the respective human polyomavirus, indicating that the BK and JC assays were detected cross-reacting antibodies. Similarly, the reactivity of the majority of human sera to SV40 was blocked by both SV40 and BK or JC, demonstrating that the SV40 reactivity of human sera is largely due to cross reacting BK and JC antibodies. In contrast, the reactivity of human sera to LPV VLPs was blocked by LPV but not by BK or JC, providing serological evidence for an un...Continue Reading

Citations

Mar 12, 2013·Nature Reviews. Microbiology·James A DeCaprio, Robert L Garcea
Sep 25, 2009·Journal of the National Cancer Institute·Joseph J CarterDenise A Galloway
Sep 2, 2011·Clinical and Vaccine Immunology : CVI·Raphael P ViscidiDaniela Rivanera
Feb 26, 2010·Journal of Clinical Microbiology·Antoine TouzéPierre Coursaget
Feb 9, 2007·Journal of Virology·Tobias AllanderBjörn Andersson
Mar 28, 2009·PLoS Pathogens·Jaime M KeanRobert L Garcea
Mar 1, 2009·Immunotherapy·Torbjörn Ramqvist, Tina Dalianis
Jul 31, 2012·Emerging Infectious Diseases·Jérôme T J NicolPierre Coursaget
Jul 14, 2012·Immunotherapy·Tina Dalianis
Sep 29, 2011·Annual Review of Pathology·Yuan Chang, Patrick S Moore
Feb 8, 2007·Emerging Infectious Diseases·Regis A Vilchez
Sep 24, 2011·Pathology Research International·Ugo MoensMarijke Van Ghelue
Apr 11, 2012·Clinical & Developmental Immunology·Serena DelbuePasquale Ferrante
May 7, 2009·Seminars in Cancer Biology·Tina DalianisRobert L Garcea
Nov 30, 2006·International Journal of Cancer. Journal International Du Cancer·Keerti V Shah
Jun 6, 2009·International Journal of Cancer. Journal International Du Cancer·Yanis L TolstovPatrick S Moore
Mar 31, 2012·Reviews in Medical Virology·Marijke Van GhelueUgo Moens
Dec 21, 2012·The Journal of General Virology·Mariet C W FeltkampAlexander E Gorbalenya
Sep 13, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Yuan ChangRobin A Weiss
Sep 9, 2006·Clinical and Vaccine Immunology : CVI·Parmjeet S RandhawaRaphael P Viscidi
May 8, 2013·Reviews in Medical Virology·Ugo MoensBernhard Ehlers
Nov 12, 2013·Journal of Medical Virology·Vojtěch SrollerSárka Němečková
Jun 21, 2013·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Muhammed Babakir-MinaMarco Ciotti
Feb 17, 2009·The Journal of General Virology·Parmjeet RandhawaNeil D Christensen
Oct 12, 2016·Microbiology Spectrum·Linda Cook
Sep 5, 2009·Journal of Virological Methods·Haruhiko MurataKeith Peden
Sep 21, 2017·Cytotherapy·Sarah I Davies, Pawel Muranski

❮ Previous
Next ❯

Related Concepts

Related Feeds

BK Virus Infection

BK virus infection is a significant complication of modern immunosuppression used in kidney transplantation. Discover the latest research on BK virus infection here.

Related Papers

International Journal of Cancer. Journal International Du Cancer
Silvia de SanjoseRaphael Viscidi
Advances in Experimental Medicine and Biology
Annika Lundstig, Joakim Dillner
Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Serena DelbuePasquale Ferrante
© 2022 Meta ULC. All rights reserved