Serological crossreactivity between Brucella abortus and Yersinia enterocolitica 0:9. III. Specificity of the in vitro antigen-specific gamma interferon test for bovine brucellosis diagnosis in experimentally Yersinia enterocolitica 0:9-infected cattle

Veterinary Microbiology
R KittelbergerC Staak

Abstract

The course of immunological reaction in 10 Yersinia enterocolitica 0:9 experimentally-infected heifers was followed using the conventional brucellosis tests complement fixation test (CFT), serum agglutination test (SAT) and brucella card test (BCT), and a recently developed Brucella antigen-specific gamma interferon (IFN-gamma) test. Initially, the animals were exposed orally to 10(10) colony-forming units (CFU) of Y. enterocolitica 0:9. Four weeks later, they were inoculated intravenously with 10(8) CFU of Y. enterocolitica 0:9 cells. After oral inoculation, the response in the conventional brucellosis tests was minimal. Only after intravenous inoculation were CFT and SAT titres and BCT reactions comparable to natural, false positive brucellosis reactors. After oral exposure the Brucellergen-stimulated release of IFN-gamma peaked at values above the cut-off stimulation index of 2.5 in 80% of the heifers. After intravenous inoculation, stimulation indices above 2.5 were present in only 10% of the animals. Two B. abortus infected control cattle showed stimulation indices of 3.1 and 3.4, and a negative control animal exhibited a stimulation index of 1.0. These findings show, in contrast to a previous study, that the Brucellergen-...Continue Reading

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Citations

Oct 16, 2007·Veterinary Research Communications·R E ThirlwallJ A Stack
Mar 9, 2010·BMC Infectious Diseases·Pilar M MuñozChristian Gortázar
Oct 8, 1999·Emerging Infectious Diseases·F GourdonJ Sirot
Jun 27, 2008·Journal of Immunological Methods·John A McGivenJudy A Stack
May 27, 2005·Journal of Applied Microbiology·S J CutlerN J Commander
May 13, 2014·Veterinary Immunology and Immunopathology·Marta Pérez-SanchoJulio Álvarez
Nov 7, 2006·The Journal of Veterinary Medical Science·Masahisa WataraiNaotaka Ishiguro
Jul 21, 2001·Tuberculosis·P R Wood, S L Jones
May 11, 2016·The Veterinary Journal·Michael P ReichelFraser I Hill
May 17, 2012·PLoS Pathogens·Raquel Conde-ÁlvarezJean-Pierre Gorvel
Oct 28, 2014·Mathematical Biosciences and Engineering : MBE·Mingtao LiYaohui Zheng

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