Abstract
The course of immunological reaction in 10 Yersinia enterocolitica 0:9 experimentally-infected heifers was followed using the conventional brucellosis tests complement fixation test (CFT), serum agglutination test (SAT) and brucella card test (BCT), and a recently developed Brucella antigen-specific gamma interferon (IFN-gamma) test. Initially, the animals were exposed orally to 10(10) colony-forming units (CFU) of Y. enterocolitica 0:9. Four weeks later, they were inoculated intravenously with 10(8) CFU of Y. enterocolitica 0:9 cells. After oral inoculation, the response in the conventional brucellosis tests was minimal. Only after intravenous inoculation were CFT and SAT titres and BCT reactions comparable to natural, false positive brucellosis reactors. After oral exposure the Brucellergen-stimulated release of IFN-gamma peaked at values above the cut-off stimulation index of 2.5 in 80% of the heifers. After intravenous inoculation, stimulation indices above 2.5 were present in only 10% of the animals. Two B. abortus infected control cattle showed stimulation indices of 3.1 and 3.4, and a negative control animal exhibited a stimulation index of 1.0. These findings show, in contrast to a previous study, that the Brucellergen-...Continue Reading
References
Apr 1, 1990·Veterinary Microbiology·Z Bercovich, E A ter Laak
Jun 1, 1989·Veterinary Immunology and Immunopathology·Z BercovichB A Bokhout
Dec 1, 1970·The Journal of Hygiene·M J Corbel, G A Cullen
Feb 15, 1984·European Journal of Biochemistry·M CaroffM B Perry
Oct 1, 1981·American Journal of Clinical Pathology·S Strobel
Dec 1, 1993·Journal of Medical Microbiology·J N LimetG Dubray
Feb 1, 1993·Journal of Biochemical and Biophysical Methods·R Kittelberger, F Hilbink
Jan 1, 1996·Veterinary Microbiology·V WeynantsJ J Letesson
Jan 1, 1996·Veterinary Research Communications·Z BercovichJ Haagsma
Dec 1, 1995·Veterinary Microbiology·R KittelbergerA Cloeckaert
Dec 1, 1995·Veterinary Microbiology·R KittelbergerK Nielsen
Oct 1, 1995·New Zealand Veterinary Journal·F HilbinkG P Ross
Citations
Oct 16, 2007·Veterinary Research Communications·R E ThirlwallJ A Stack
Nov 5, 2002·Veterinary Microbiology·Jacques GodfroidBruno Garin-Bastuji
May 5, 1999·Veterinary Microbiology·B Garin-BastujiJ J Fontaine
Nov 9, 2005·Clinical and Diagnostic Laboratory Immunology·Mindy L RawlinsChristine M Litwin
Mar 9, 2010·BMC Infectious Diseases·Pilar M MuñozChristian Gortázar
Oct 8, 1999·Emerging Infectious Diseases·F GourdonJ Sirot
Jul 11, 1998·Preventive Veterinary Medicine·R PouillotM Sanaa
Dec 3, 2015·Vaccine·Liangquan ZhuXun Suo
Jun 27, 2008·Journal of Immunological Methods·John A McGivenJudy A Stack
May 27, 2005·Journal of Applied Microbiology·S J CutlerN J Commander
May 13, 2014·Veterinary Immunology and Immunopathology·Marta Pérez-SanchoJulio Álvarez
Nov 7, 2006·The Journal of Veterinary Medical Science·Masahisa WataraiNaotaka Ishiguro
Jul 21, 2001·Tuberculosis·P R Wood, S L Jones
Feb 3, 2007·Veterinary Immunology and Immunopathology·U Riber, G Jungersen
May 11, 2016·The Veterinary Journal·Michael P ReichelFraser I Hill
May 6, 2016·International Journal of Molecular Sciences·Gamal WarethJayaseelan Murugaiyan
May 17, 2012·PLoS Pathogens·Raquel Conde-ÁlvarezJean-Pierre Gorvel
Nov 22, 2013·PloS One·Karolina SkorekKatarzyna Brzostek
Sep 6, 2005·Veterinary Immunology and Immunopathology·K NielsenJ Godfroid
Oct 28, 2014·Mathematical Biosciences and Engineering : MBE·Mingtao LiYaohui Zheng
Mar 31, 2011·Biotechnology and Applied Biochemistry·Wen-xing LiuXiang-rui Li
Jun 21, 2005·Veterinary Immunology and Immunopathology·M D DipteeG T Fosgate
Jul 11, 2003·Clinical and Diagnostic Laboratory Immunology·Janchivdorj ErdenebaatarToshikazu Shirahata